The sulfated polysaccharides are of study interest due to their high structural diversity and broad spectrum of biological activity including antitumor properties. In this paper, we report on the structural analysis of sulfated O-specific polysaccharide (OPS) and in vitro anticancer activity of O-deacylated lipopolysaccharide (DPS) of the marine-derived bacterium Poseidonocella sedimentorum KMM 9023^T achieved by a multidisciplinary approach (chemical analysis, NMR, MS, and bioassay). The OPS is shown to include two rare monosaccharide derivatives: 3-deoxy-9-O-methyl-D-glycero-D-galacto-non-2-ulosonic acid (Kdn9Me) and 3-O-acetyl-2-O-sulfate-D-glucuronic acid (D-GlcA2S3Ac). The structure of polysaccharide moiety of a previously unknown carbohydrate-containing biopolymer is established:

→4)-α-Kdnp9Me-(2→4)-α-D-GlcpA2S3Ac-(1→.

From a biological point of view, we demonstrate that DPS of the P. sedimentorum KMM 9023^T has no cytotoxicity and inhibits colony formation of human HT-29, MCF-7 and SK-MEL-5 cells in a dose-dependent manner. The investigated polysaccharide is the second glycan isolated from the bacteria of the genus Poseidonocella: previously we studied the OPS of P. pacifica KMM 9010^T (Kokoulin et al., 2017). Both polysaccharides are sulfated and contain rare residues of ulosonic acids. Thus, obtained findings provide a new knowledge about kinds and antitumor properties of sulfated polysaccharides and can be a starting point for further investigations of mechanisms of anticancer action of carbohydrate-containing biopolymers from marine Gram-negative bacteria.

硫酸化多糖由于其高度的结构多样性和广泛的生物活性（包括抗肿瘤特性）而受到研究的兴趣。在本文中，我们报告了通过多学科方法（化学方法）获得的海洋衍生细菌Poseidonocella depositorum KMM 9023 ^T的硫酸化O特异性多糖（OPS）的结构分析和O脱酰脂多糖（DPS）的体外抗癌活性。分析，NMR，MS和生物分析）。显示OPS包含两种稀有的单糖衍生物：3-脱氧-9 - O-甲基-D-甘油-D-半乳糖基非-2-乌磺酸（Kdn9Me）和3 - O-乙酰基-2- O-硫酸盐-D-葡萄糖醛酸（D-GlcA2S3Ac）。建立了先前未知的含碳水化合物的生物聚合物的多糖部分的结构：

→4）-α-Kdnp9Me-（2→4）-α-D-GlcpA2S3Ac-（1→。

从生物学的角度来看，我们证明了体育沉积物KMM 9023 ^T的DPS没有细胞毒性，并以剂量​​依赖的方式抑制了人HT-29，MCF-7和SK-MEL-5细胞的集落形成。研究的多糖是从波塞冬菌属细菌中分离出的第二种聚糖：以前我们研究过P. pacifica KMM 9010 ^T的OPS（Kokoulin et al。，2017）。两种多糖都被硫酸化并含有罕见的ulosonic acid残基。因此，获得的发现提供了关于硫酸化多糖的种类和抗肿瘤特性的新知识，并且可以作为进一步研究海洋革兰氏阴性细菌中的含糖生物聚合物的抗癌作用机理的起点。