In diabetes patients, hyperglycemia causes many vascular disorders. To clarify the effects of water extract of Brazilian green propolis (WEP) against high glucose-induced vascular disorders, we investigated using zebrafish-based vasculopathy model. Zebrafish larvae, which is transparent and small, is an ideal model organism to detect changes of the vascular structure. Zebrafish larvae were exposed to 200 mM glucose and WEP solution from 2 h post fertilization (hpf) to 3 days post fertilization (dpf). The WEP treatment normalized high glucose-induced morphological abnormality of the larvae, hyaloid vessels, and cerebral vessels. The WEP treatment partly suppressed the elevation of glucose levels and mRNA levels of p27 in tissues by exposure to high glucose content. Furthermore, we found that chlorogenic acid, which is one of the main constituents of WEP, also normalized high glucose-induced morphological abnormality. Our findings indicated that WEP improves vascular integrity in the central nervous system under high glucose conditions.

在糖尿病患者中，高血糖症会引起许多血管疾病。为了阐明巴西绿蜂胶（WEP）的水提取物对高糖诱导的血管疾病的作用，我们使用基于斑马鱼的血管病变模型进行了调查。斑马鱼幼虫是透明的，很小，是检测血管结构变化的理想模型生物。从受精后2小时（hpf）到受精后3天（dpf），将斑马鱼的幼虫暴露于200 mM葡萄糖和WEP溶液中。WEP治疗可以使高糖诱导的幼虫，玻璃样血管和脑血管的形态异常正常化。WEP处理通过暴露于高葡萄糖含量，部分抑制了组织中葡萄糖水平和p27 mRNA水平的升高。此外，我们发现绿原酸 它是WEP的主要成分之一，也使高糖诱导的形态异常正常化。我们的研究结果表明，WEP可改善高糖条件下中枢神经系统的血管完整性。