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On-demand quantitative SERS bioassays facilitated by surface-tethered ratiometric probes†
Chemical Science ( IF 7.6 ) Pub Date : 2018-08-31 00:00:00 , DOI: 10.1039/c8sc03263g Kun Zhang 1, 2, 3, 4, 5 , Yuning Wang 1, 2, 3, 4, 5 , Meiling Wu 6, 7, 8, 9 , Yujie Liu 1, 2, 3, 4, 5 , Dongyun Shi 6, 7, 8, 9 , Baohong Liu 1, 2, 3, 4, 5
Chemical Science ( IF 7.6 ) Pub Date : 2018-08-31 00:00:00 , DOI: 10.1039/c8sc03263g Kun Zhang 1, 2, 3, 4, 5 , Yuning Wang 1, 2, 3, 4, 5 , Meiling Wu 6, 7, 8, 9 , Yujie Liu 1, 2, 3, 4, 5 , Dongyun Shi 6, 7, 8, 9 , Baohong Liu 1, 2, 3, 4, 5
Affiliation
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Reliable and user-friendly sensing of target analytes in complex biofluids is of fundamental importance to biological science and medicine. Surface-enhanced Raman spectroscopy (SERS) has proven to be capable of detecting molecules with high sensitivity, but achieving robust quantitative detection remains a challenge mainly because of the severe signal fluctuation at electromagnetic hot spots. Here, we describe an on-demand and quantitative SERS strategy for metabolite profiling based on a chip-based sensing device that adopts stable and surface-tethered small-molecule probes as Raman reporters. These probes with a ratiometric response allow for sensitive and reproducible SERS detection by offering an internal calibration to correct the signal fluctuation caused by the spatiotemporal variation of assay conditions. Meanwhile, the chip-based sensing scheme makes time-separated on-demand detection possible. Ultimately, due to the flexibility in choosing diverse ratiometric Raman probes, we expect the proposed quantitative SERS sensing concept to be useful for studies in the fields of cell biology and clinical diagnosis.
中文翻译：
表面束缚式比例探针促进按需定量SERS生物测定†
对复杂生物流体中的目标分析物进行可靠且用户友好的传感对生物科学和医学至关重要。表面增强拉曼光谱（SERS）已被证明能够以高灵敏度检测分子，但是实现强大的定量检测仍然是一个挑战，主要是因为电磁热点处的信号波动很大。在这里，我们描述了基于芯片的传感设备的代谢物谱分析的按需定量SERS策略，该芯片采用稳定且表面束缚的小分子探针作为拉曼报告分子。这些具有比例响应的探头可通过提供内部校准来校正由检测条件的时空变化引起的信号波动，从而实现灵敏且可重复的SERS检测。同时，基于芯片的传感方案使按时间进行按需检测成为可能。最终，由于可以灵活地选择多种比例拉曼探针，我们希望提出的定量SERS传感概念对细胞生物学和临床诊断领域的研究有用。
更新日期：2018-08-31
中文翻译：
表面束缚式比例探针促进按需定量SERS生物测定†
对复杂生物流体中的目标分析物进行可靠且用户友好的传感对生物科学和医学至关重要。表面增强拉曼光谱（SERS）已被证明能够以高灵敏度检测分子，但是实现强大的定量检测仍然是一个挑战，主要是因为电磁热点处的信号波动很大。在这里，我们描述了基于芯片的传感设备的代谢物谱分析的按需定量SERS策略，该芯片采用稳定且表面束缚的小分子探针作为拉曼报告分子。这些具有比例响应的探头可通过提供内部校准来校正由检测条件的时空变化引起的信号波动，从而实现灵敏且可重复的SERS检测。同时，基于芯片的传感方案使按时间进行按需检测成为可能。最终，由于可以灵活地选择多种比例拉曼探针，我们希望提出的定量SERS传感概念对细胞生物学和临床诊断领域的研究有用。
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