Parallel, Large-Scale, and Long Synthetic Oligodeoxynucleotide Purification Using the Catching Full-Length Sequence by Polymerization Technique,Organic Process Research & Development

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Parallel, Large-Scale, and Long Synthetic Oligodeoxynucleotide Purification Using the Catching Full-Length Sequence by Polymerization Technique
Organic Process Research & Development ( IF 3.1 ) Pub Date : 2018-08-31 00:00:00 , DOI: 10.1021/acs.oprd.8b00209
Dhananjani N A M Eriyagama ₁ , Shahien Shahsavari ₁ , Bhaskar Halami ₁ , Bao-Yuan Lu ₂ , Fengping Wei ₃ , Shiyue Fang ₁

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The catching by polymerization synthetic oligodeoxynucleotide (ODN) purification technique was shown to be potentially suitable for high-throughput purification by the purification of 12 ODNs simultaneously, to be convenient for large-scale purification by purification at the 60 μmol synthesis scale, and to be highly powerful for purification of long ODNs by purification of ODNs as long as 303-mers. LC–MS analysis indicated that the ODNs purified with the technique have excellent purity.

中文翻译：

使用聚合技术捕获全长序列进行平行、大规模、长合成寡脱氧核苷酸纯化

聚合捕获合成寡脱氧核苷酸（ODN）纯化技术被证明可能适用于同时纯化12个ODN的高通量纯化，便于在60μmol合成规模下进行大规模纯化，并且通过纯化长达 303-mers 的 ODN，可高效纯化长 ODN。LC-MS分析表明，用该技术纯化的ODN具有优异的纯度。

更新日期：2018-08-31

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