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Rationally designing hydrophobic UiO-66 support for the enhanced enzymatic performance of immobilized lipase
Green Chemistry ( IF 9.3 ) Pub Date : 2018-08-30 , DOI: 10.1039/c8gc01284a Yingli Hu 1, 2, 3, 4, 5 , Lingmei Dai 1, 2, 3, 4, 5 , Dehua Liu 1, 2, 3, 4, 5 , Wei Du 1, 2, 3, 4, 5
Green Chemistry ( IF 9.3 ) Pub Date : 2018-08-30 , DOI: 10.1039/c8gc01284a Yingli Hu 1, 2, 3, 4, 5 , Lingmei Dai 1, 2, 3, 4, 5 , Dehua Liu 1, 2, 3, 4, 5 , Wei Du 1, 2, 3, 4, 5
Affiliation
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The immobilization of Aspergillus niger lipase (ANL) on UiO-66 (a typical MOF), the effect of support hydrophobicity on lipase's immobilization as well as the enzymatic performance for biodiesel preparation was explored in this enzyme/MOF system. First, the hydrophobic UiO-66 was obtained by polydimethylsiloxane (PDMS) coating, and then used as the immobilization carrier for physically adsorbing ANL through hydrophobic interactions. It was found that the properties of immobilized ANL were remarkably enhanced through hydrophobic modification of UiO-66. ANL immobilized on hydrophobic UiO-66 presented much higher enzymatic activity and activity recovery compared with those of ANL on pristine UiO-66. In the catalysis of lipase-mediated biodiesel production, ANL immobilized on hydrophobic UiO-66-PDMS-6 h performed at a faster reaction rate and gave a much higher yield than its counterpart. A biodiesel yield of 88% was afforded by ANL/UiO-66-PDMS-6 h at 24 h, and the immobilized lipase demonstrated a rather good reusability, with 83.0% activity remaining after ten successive operations.
中文翻译：
合理设计疏水性UiO-66载体，以提高固定化脂肪酶的酶促性能
黑曲霉的固定化在此酶/ MOF系统中，研究了UiO-66（典型的MOF）上的脂肪酶（ANL），载体疏水性对脂肪酶固定化的影响以及生物柴油制备的酶促性能。首先，通过聚二甲基硅氧烷（PDMS）涂层获得疏水性UiO-66，然后用作通过疏水性相互作用物理吸附ANL的固定载体。发现通过UiO-66的疏水改性显着提高了固定化的ANL的性质。固定在疏水UiO-66上的ANL与原始UiO-66上的ANL相比具有更高的酶促活性和活性恢复。在脂肪酶介导的生物柴油生产的催化中，固定在疏水性UiO-66-PDMS-6 h上的ANL以更快的反应速率进行操作，并且比其对应物具有更高的产率。ANL / UiO-66-PDMS-6 h在24 h时可提供88％的生物柴油收率，固定化的脂肪酶具有相当好的可重复使用性，在连续十次操作后仍具有83.0％的活性。
更新日期：2018-10-01
中文翻译：
合理设计疏水性UiO-66载体，以提高固定化脂肪酶的酶促性能
黑曲霉的固定化在此酶/ MOF系统中，研究了UiO-66（典型的MOF）上的脂肪酶（ANL），载体疏水性对脂肪酶固定化的影响以及生物柴油制备的酶促性能。首先，通过聚二甲基硅氧烷（PDMS）涂层获得疏水性UiO-66，然后用作通过疏水性相互作用物理吸附ANL的固定载体。发现通过UiO-66的疏水改性显着提高了固定化的ANL的性质。固定在疏水UiO-66上的ANL与原始UiO-66上的ANL相比具有更高的酶促活性和活性恢复。在脂肪酶介导的生物柴油生产的催化中，固定在疏水性UiO-66-PDMS-6 h上的ANL以更快的反应速率进行操作，并且比其对应物具有更高的产率。ANL / UiO-66-PDMS-6 h在24 h时可提供88％的生物柴油收率，固定化的脂肪酶具有相当好的可重复使用性，在连续十次操作后仍具有83.0％的活性。
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