A probe of 8,8′-((1Z,1′Z)-(((propane-1,3-diylbis(oxy))bis(2,1-phenylene))bis(azanylylidene))bis(methanylylidene))bis(7-hydroxy-4-methyl-2H-chromen-2-one) (H₂L) was able to recognize Zn²⁺via its turn-on emission at 510 nm in a MeCN–water (9 : 1, v/v) solution in the presence of a large number of biologically important cations with a very low limit of detection (LOD), namely 11 nM, which meant it was about 3500 times more sensitive than the WHO recommended permissible standard for zinc in drinking water (3 ppm). The probe, H₂L, showed solvent polarity sensitive absorption and emission properties and the λ_max showed negative solvatochromism. The composition of the complex, [ZnL], was established by a Job's plot, HR-MS data, and single-crystal X-ray diffraction measurements. The selectivity and sensitivity of the probe, H₂L, was successfully applied to the detection of intracellular Zn²⁺ in human hepato-cellular-carcinoma (HepG2) cells. H₂L is also applicable for the analysis of Zn²⁺ ions in tap water.

中文翻译：

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新型香豆素支架通过荧光光谱技术 对锌（ii）的高度选择性和灵敏识别及其在活细胞中的应用†

的8,8探针' - （（1个Ž，1' Ž） - （（（丙烷-1,3-二基双（氧基））双（2,1-亚苯基））二（azanylylidene））双（methanylylidene） ）双（7-羟基-4-甲基-2 H-铬-2--2-酮）（H ₂ L）能够通过在510 nm的MeCN-水中（9：1 ）识别Zn ²⁺。，v / v）溶液中存在大量生物学上重要的阳离子，且其检测限（LOD）极低，即11 nM，这意味着其灵敏度比WHO建议的锌中允许的锌标准高3500倍饮用水（3 ppm）。探针H ₂ L表现出对溶剂极性敏感的吸收和发射特性，而λ_max显示阴性溶剂溶变色。配合物[ZnL]的组成是通过Job's图，HR-MS数据和单晶X射线衍射测量确定的。探针H ₂ L的选择性和灵敏度已成功应用于检测人肝细胞癌（HepG2）细胞中的细胞内Zn ²⁺。H ₂ L还适用于分析自来水中的Zn ²⁺离子。