The present study attempted to develop a fast and sensitive ultrasound-assisted-dispersive-micro-solid phase extraction (UA-D-μ-SPE) method for the separation and preconcentration of albendazole (ABZ) from plasma and urine samples, using a molecularly imprinted polymer (MIP) coupled with high-performance liquid chromatography-UV (HPLC-UV). An MIP was selected as a sorbent, and the main factors viz. pH, MIP mass, sonication time, and eluent volume were optimized using CCD-RSM (central composite design-response surface methodology). Under the optimum conditions, the method showed the detection limit of 1.46, quantification of 4.86, and linear range of 10.0–6000.0 ng mL⁻¹. The relative standard deviation and the relative recoveries of ABZ from spiked plasma and urine samples at five concentration levels (20, 50, 100, 500, and 1000 ng mL⁻¹) were in the ranges of 1.43–5.21% and 92.48–101.40%, respectively. The kinetic sorption curve indicated that six min was sufficient to achieve sorption equilibrium for MIPNPs (MIP nanoparticles). The maximum sorption capacity was 34.9 mg g⁻¹. All these results strongly support the high efficiency, selectivity, and sensitivity of the developed method for determining ABZ in plasma and urine samples.

中文翻译：

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分子印迹聚合物与高效液相色谱-UV结合用于测定血浆和尿液样品中的阿苯达唑：CCD-RSM设计†

本研究试图开发一种快速灵敏的超声辅助分散微固相萃取（UA-D-μ-SPE）方法，用于从血浆和尿液样品中分离和预浓缩阿苯达唑（ABZ），使用分子印迹聚合物（MIP）结合高效液相色谱-UV（HPLC-UV）。选择了MIP作为吸附剂，主要因素即为吸附剂。使用CCD-RSM（中央复合设计-响应表面方法）优化了pH，MIP质量，超声处理时间和洗脱液体积。在最佳条件下，该方法的检出限为1.46，定量为4.86，线性范围为10.0–6000.0 ng mL ^-1。在五个浓度水平（20、50、100、500和1000 ng mL ^-1）下，加标血浆和尿液样品中ABZ的相对标准偏差和相对回收率在1.43–5.21％和92.48–101.40％范围内， 分别。动力学吸附曲线表明六分钟足以达到MIPNP（MIP纳米颗粒）的吸附平衡。最大吸附容量为34.9mg g ^-1。所有这些结果都强烈支持所开发的测定血浆和尿液样品中ABZ的方法的高效性，选择性和敏感性。