A Synthetic Microbial Operational Amplifier,ACS Synthetic Biology

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A Synthetic Microbial Operational Amplifier
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2018-08-28 00:00:00 , DOI: 10.1021/acssynbio.8b00138
Ji Zeng , Jonathan Teo ₁ , Areen Banerjee , Timothy W Chapman , Jaewook Kim , Rahul Sarpeshkar

Affiliation

Synthetic biology has created oscillators, latches, logic gates, logarithmically linear circuits, and load drivers that have electronic analogs in living cells. The ubiquitous operational amplifier, which allows circuits to operate robustly and precisely has not been built with biomolecular parts. As in electronics, a biological operational-amplifier could greatly improve the predictability of circuits despite noise and variability, a problem that all cellular circuits face. Here, we show how to create a synthetic three-stage inducer-input operational amplifier with a fast CRISPR-based differential-input push–pull stage, a slow transcription-and-translation amplification stage, and a fast-enzymatic output stage. Our “Bio-OpAmp” uses only 5 proteins including dCas9. It expands the toolkit of fundamental analog circuits in synthetic biology and provides a simple circuit motif for robust and precise molecular homeostasis.

中文翻译：

合成微生物运算放大器

合成生物学已经创造了振荡器、锁存器、逻辑门、对数线性电路和负载驱动器，它们在活细胞中具有电子类似物。无处不在的运算放大器可以让电路稳健而精确地运行，但它并不是用生物分子部件构建的。与电子学一样，生物运算放大器可以极大地提高电路的可预测性，尽管噪声和可变性是所有细胞电路都面临的问题。在这里，我们展示了如何创建一个合成的三级诱导器输入运算放大器，具有基于 CRISPR 的快速差分输入推挽级、慢速转录和翻译放大级以及快速酶输出级。我们的“Bio-OpAmp”仅使用 5 种蛋白质，包括 dCas9。它扩展了合成生物学中基本模拟电路的工具包，并为稳健而精确的分子稳态提供了简单的电路基序。

更新日期：2018-08-28

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