Abstract A method for the determination of carbaryl in vegetable samples was developed employing peroxidase enzyme extracts from newly excised rubber tree bark strips as a catalyst. The solid phase extraction method QuEChERS was utilized for sample preparation followed by dispersive liquid-liquid microextraction (DLLME) to increase the concentration of the analyte before analysis by UV–Visible spectrophotometry. Parameters influencing this enzymatic development method and efficiency of DLLME were carefully studied. Under selected conditions, absorbance was in proportion to carbaryl concentration in the range of 0.1–3.0 mg L−1 with linear regression (r2) of 0.9999. Limit of detection (LOD) and limit of quantitation (LOQ) were 0.06 mg L−1 and 0.25 mg L−1, respectively. Relative standard deviation (RSD) values for the samples were 2.51–4.06% (n = 7). Good recoveries of carbaryl were obtained in the range of 83–118%. The proposed method proved rapid, reliable and selective for determination of carbaryl in vegetable samples.

中文翻译：

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基于橡胶树皮过氧化物酶反应分光光度法测定甲萘威的新方法

摘要 以新摘取的橡胶树皮条的过氧化物酶提取物为催化剂，建立了测定蔬菜样品中甲萘威的方法。固相萃取方法 QuEChERS 用于样品制备，然后采用分散液-液微萃取 (DLLME) 以增加分析物的浓度，然后再通过紫外-可见分光光度法进行分析。仔细研究了影响这种酶促开发方法和 DLLME 效率的参数。在选定条件下，吸光度与甲萘威浓度成正比，范围为 0.1–3.0 mg L-1，线性回归 (r2) 为 0.9999。检测限 (LOD) 和定量限 (LOQ) 分别为 0.06 mg L-1 和 0.25 mg L-1。样品的相对标准偏差 (RSD) 值为 2.51–4.06% (n = 7)。在 83-118% 的范围内获得了良好的甲萘威回收率。事实证明，该方法可快速、可靠且具有选择性，可用于测定蔬菜样品中的甲萘威。