Activation of Split RNA Aptamers: Experiments Demonstrating the Enzymatic Synthesis of Short RNAs and Their Assembly As Observed by Fluorescent Response,Journal of Chemical Education

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Activation of Split RNA Aptamers: Experiments Demonstrating the Enzymatic Synthesis of Short RNAs and Their Assembly As Observed by Fluorescent Response
Journal of Chemical Education ( IF 2.5 ) Pub Date : 2018-08-28 00:00:00 , DOI: 10.1021/acs.jchemed.7b00759
Sameer Sajja ₁ , Morgan Chandler ₁ , Caryn D. Striplin ₁ , Kirill A. Afonin ₁

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With the advancement of ribonucleic acid (RNA) research in the field of biochemistry, ensuring that undergraduate students have both the theoretical and practical knowledge of current, cutting-edge experimental techniques is of marked importance. Many current biochemistry experiments demonstrate various basic molecular biological techniques including isolation and quantification of nucleic acids, protein gel electrophoresis, and DNA amplification reactions. While covering a wide base of skills, there is an underutilization of RNA as a pedagogical tool. This multistep laboratory experiment introduces students to the emerging field of RNA nanotechnology and to the RNA assembly and subsequent conditional activations of preprogrammed functionalities such as fluorescence. To achieve this, a sequence of standard molecular techniques such as PCR, DNA purification, RNA transcription and purification, RNA quantification, and self-assembly followed by analysis with electrophoretic mobility shift assays and spectroscopic quantification are carried out in a semester-long curriculum offered to undergraduate and lower-level graduate students following a prerequisite biochemistry lab course.

中文翻译：

分裂的RNA适体的激活：实验证明短RNA的酶促合成及其组装如荧光反应所观察。

随着生物化学领域核糖核酸（RNA）研究的发展，确保大学生掌握当前的理论知识和实践知识，尖端的实验技术至关重要。当前许多生物化学实验证明了各种基本的分子生物学技术，包括核酸的分离和定量，蛋白质凝胶电泳和DNA扩增反应。虽然涵盖了广泛的技能基础，但RNA作为一种教学工具的利用不足。这个多步骤实验室实验向学生介绍了RNA纳米技术的新兴领域，RNA组装以及随后对诸如荧光之类的预编程功能的条件激活。为此，一系列标准分子技术（例如PCR，

更新日期：2018-08-28

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