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Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-08-27 00:00:00 , DOI: 10.1021/acs.jafc.8b02708 Xianping Fang 1, 2 , Ya Xin 3 , Zheliang Sheng 4 , Hui Liu 3 , Aili Jiang 2 , Fang Wang 5 , Jian Yang 1 , Xiaojun Xi 2 , Qian Zha 2 , Liqing Zhang 2 , Liangying Dai 6 , Chengqi Yan 5 , Jianping Chen 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-08-27 00:00:00 , DOI: 10.1021/acs.jafc.8b02708 Xianping Fang 1, 2 , Ya Xin 3 , Zheliang Sheng 4 , Hui Liu 3 , Aili Jiang 2 , Fang Wang 5 , Jian Yang 1 , Xiaojun Xi 2 , Qian Zha 2 , Liqing Zhang 2 , Liangying Dai 6 , Chengqi Yan 5 , Jianping Chen 1
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The various post-translational modifications (PTMs) of plant proteins have important regulatory roles in development. We therefore examined various modified proteins from strawberry stigmata and found that succinylation of lysine residues was the most abundant type of modification. We then subjected proteins from strawberry stigmata to an efficient enrichment method for succinylated peptides and identified 200 uniquely succinylated lysines (Suks) in 116 proteins. A bioinformatics analysis revealed that these proteins are involved in important biological processes, including stress responses, vesicular transport, and energy metabolism. Proteomics, combined with immunoprecipitation and immunoblotting, revealed an obvious increase in succinylation of the assembly polypeptide 2 (AP2) and clathrin from 0.5 to 2 h after pollination, suggesting that succinylation is involved in the recognition of pollen–stigma signaling substances and vesicular transport. These results suggest that AP2/clathrin-mediated vesicular transport processes are regulated by lysine succinylation during pollen recognition.
中文翻译：
草莓柱头赖氨酸琥珀酰化的系统鉴定与分析
植物蛋白的各种翻译后修饰（PTM）在发育中具有重要的调控作用。因此，我们检查了草莓柱头的各种修饰蛋白，发现赖氨酸残基的琥珀酰化是修饰最丰富的类型。然后，我们对草莓柱头处的蛋白质进行了琥珀酰化肽的有效富集方法，并在116种蛋白质中鉴定出200种独特的琥珀酰化赖氨酸（Suks）。生物信息学分析表明，这些蛋白质参与重要的生物过程，包括应激反应，囊泡运输和能量代谢。蛋白质组学与免疫沉淀和免疫印迹相结合，揭示了组装多肽2（AP2）和网格蛋白的琥珀酰化从授粉后0.5到2 h明显增加，提示琥珀酰化参与花粉-柱头信号物质和水泡运输的识别。这些结果表明在花粉识别过程中，AP2 / clathrin介导的囊泡运输过程受赖氨酸琥珀酰化作用的调节。
更新日期：2018-08-27
中文翻译：
草莓柱头赖氨酸琥珀酰化的系统鉴定与分析
植物蛋白的各种翻译后修饰（PTM）在发育中具有重要的调控作用。因此，我们检查了草莓柱头的各种修饰蛋白，发现赖氨酸残基的琥珀酰化是修饰最丰富的类型。然后，我们对草莓柱头处的蛋白质进行了琥珀酰化肽的有效富集方法，并在116种蛋白质中鉴定出200种独特的琥珀酰化赖氨酸（Suks）。生物信息学分析表明，这些蛋白质参与重要的生物过程，包括应激反应，囊泡运输和能量代谢。蛋白质组学与免疫沉淀和免疫印迹相结合，揭示了组装多肽2（AP2）和网格蛋白的琥珀酰化从授粉后0.5到2 h明显增加，提示琥珀酰化参与花粉-柱头信号物质和水泡运输的识别。这些结果表明在花粉识别过程中，AP2 / clathrin介导的囊泡运输过程受赖氨酸琥珀酰化作用的调节。
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