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Identification of meat from yak and cattle using SNP markers with integrated allele-specific polymerase chain reaction–capillary electrophoresis method
Meat Science ( IF 5.7 ) Pub Date : 2018-08-25 , DOI: 10.1016/j.meatsci.2018.08.019
Jie Zhao , Zhenzhen Xu , Ailiang Chen , Xinyong You , Yan Zhao , Wenjing He , Luyao Zhao , Shuming Yang

Correct species identification is necessary for meat product quality assurance. Genetic markers are useful tools to accurately determine the animal source of meat products. To better protect yak meat and its products, ten single nucleotide polymorphism (SNP) loci from bovine meat-related genes and one positive control primer set were selected to discriminate meat sourced from yak or cattle. A multiplex allele-specific polymerase chain reaction and capillary electrophoresis method was employed for SNP genotyping. The samples were identified as from yak or cattle based on the number of amplicons. Yak samples generally produced one to three amplicon peaks, and the cattle samples yielded five or more peaks in the electropherogram. This method could be useful to distinguish yak meat from cattle beef, and would protect consumers and yak meat producers.



中文翻译:

结合等位基因特异性聚合酶链反应-毛细管电泳法使用SNP标记鉴定from牛和牛的肉

正确的种类识别对于保证肉制品质量是必要的。遗传标记是准确确定肉制品动物来源的有用工具。为了更好地保护牛肉及其产品,选择了十个来自牛肉相关基因的单核苷酸多态性(SNP)位点和一个阳性对照引物组,以区分源自牛或牛的肉。采用多重等位基因特异性聚合酶链反应和毛细管电泳方法进行SNP基因分型。根据扩增子的数量将样品鉴定为来自from牛或牛。k牛样品通常在电泳图中产生1-3个扩增子峰,而牛样品则产生5个或更多峰。这种方法可能有助于区分ak牛肉和牛牛肉,并可以保护消费者和牛肉生产者。

更新日期:2018-08-25
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