Mycophenolic acid (MPA) is a secondary metabolite of Penicillium species with diverse biological properties and has clinical applications mainly as an immunosuppressive agent. Production of MPA was carried out using Penicillium brevicompactum (DSM 2215) by solid-state fermentation (SSF). Six different carbon sources (wheat bran, rice husk, rice bran, potato peel and rice husk - rice bran, rice bran-potato peel mixture) were examined by SSF in order to obtain the highest yield of mycophenolic acid. Rice bran-potato peel mixture was determined as the best solid substrate and used in scale up studies which was applied in the tray bioreactor. Supercritical CO₂ and soxhlet extractions were analyzed for the recovery of MPA from the fermentation broth. The highest recovery (0.47 g MPA/100 g substrate) was obtained by supercritical CO₂ at 30 MPa, 50 °C at a CO₂ flow rate of 15 g/min with 10% ethanol as co-solvent for 3 h. The results obtained by the scale-up in bioreactor and optimization of supercritical CO₂ entrained with ethanol can provide basis for the production of the immunosuppresant compound on industrial scale.

麦考酚酸（MPA）是青霉菌种的次生代谢产物，具有多种生物学特性，主要作为免疫抑制剂在临床上得到应用。MPA的生产是使用短小青霉（DSM 2215）通过固态发酵（SSF）进行的。SSF检测了六种不同的碳源（麦麸，稻壳，米糠，马铃薯皮和稻壳-米糠，米糠-马铃薯皮混合物），以获得最高的麦考酚酸收率。米糠-马铃薯皮的混合物被确定为最佳的固体底物，并用于规模研究中，该研究已应用于托盘生物反应器中。超临界CO ₂分析索氏提取物并从发酵液中回收MPA。通过在30 MPa，50°C下以15 g / min的CO ₂流速和10％乙醇作为助溶剂的超临界CO ₂进行3 h可获得最高的回收率（0.47 g MPA / 100 g底物）。通过在生物反应器中扩大规模并优化乙醇夹带的超临界CO _2所获得的结果可为工业规模生产免疫抑制剂化合物提供基础。