Edible bird's nest (EBN) is a well-known and precious traditional Chinese herbal material (CHM). Because of this, preventing the adulteration of EBN efficiently and precisely is crucial to protect consumers' interests and health. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for the detection of EBN using specifically designed LAMP primers. The results demonstrated that the identification of EBN by LAMP assay was specific and rapid (within 1 h). It had no cross-reaction with EBN adulterants, including white fungus, egg white and pig skin, in different ratios. The relative detection limit was 0.01% EBN in the adulterants. Moreover, the sensitivity of LAMP in authenticating EBN was 10-8 μg, it showed higher sensitivity than that of conventional PCR with 105 fold. When genomic DNAs extracted from boiled or steamed EBN samples were used as templates, LAMP for EBN detection was not affected and was reproducible after heat processing. In conclusion, the LAMP assay established herein could be applicable for authenticating EBN and for identifying commercial EBN products in herbal markets.

中文翻译：

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环介导等温扩增 (LAMP) 法快速灵敏地检测加工食品中的燕窝 (Aerodramus fuciphagus)

燕窝（EBN）是一种著名的名贵中药材（CHM）。正因为如此，有效、准确地防止 EBN 掺假对于保护消费者的利益和健康至关重要。在这项研究中，开发了一种环介导的等温扩增 (LAMP) 测定法，用于使用专门设计的 LAMP 引物检测 EBN。结果表明，通过 LAMP 法鉴定 EBN 具有特异性和快速性（1 h 内）。与不同比例的银耳、蛋清、猪皮等EBN掺杂物无交叉反应。掺杂物的相对检测限为 0.01% EBN。此外，LAMP鉴定EBN的灵敏度为10-8 μg，比常规PCR灵敏度高105倍。当使用从煮沸或蒸煮的 EBN 样品中提取的基因组 DNA 作为模板时，用于 EBN 检测的 LAMP 不受影响，并且在热处理后可重现。总之，本文建立的 LAMP 测定可适用于鉴定 EBN 和识别草药市场中的商业 EBN 产品。