Comparison of NAD with NADP‐dependent Glutamate Dehydrogenase, and CNT with rGO‐modified Electrodes, for the Construction of Glutamate Sensors,Electroanalysis

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Comparison of NAD with NADP‐dependent Glutamate Dehydrogenase, and CNT with rGO‐modified Electrodes, for the Construction of Glutamate Sensors
Electroanalysis ( IF 2.7 ) Pub Date : 2018-08-22 , DOI: 10.1002/elan.201800160
Katsuhiko Sato _{1,

2} , Toshio Kamijo ₁ , Shigehiro Takahashi ₃ , Takaya Sato ₁

Affiliation

Glutamate sensors were prepared using nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP)‐dependent glutamate dehydrogenase (GDH). Glutamate was determined from the anodic oxidation current of NADH and NADPH generated by the enzymatic reaction of GDH. These two types of GDH enzymes were deposited separately on carbon nanotube (CNT) and reduced graphene oxide (rGO)‐modified glassy carbon electrodes, in which CNT and rGO were used to improve the oxidation capacity of the electrodes. An anodic peak current in response to 1 mM glutamate of glutamate sensors comprising each of these four combinations were compared under the same conditions. The glutamate response was the highest when NAD‐dependent GDH was deposited on the rGO‐modified electrode.

中文翻译：

NAD与依赖NADP的谷氨酸脱氢酶的比较以及CNT与rGO修饰的电极在谷氨酸传感器构建中的比较

使用烟酰胺腺嘌呤二核苷酸（NAD）和烟酰胺腺嘌呤二核苷酸磷酸（NADP）依赖性谷氨酸脱氢酶（GDH）制备谷氨酸传感器。由NADH和NADPH的阳极氧化电流确定谷氨酸，该NADH和NADPH是由GDH的酶促反应产生的。这两种GDH酶分别沉积在碳纳米管（CNT）和还原型氧化石墨烯（rGO）修饰的玻璃碳电极上，其中CNT和rGO用于提高电极的氧化能力。在相同条件下比较了响应于包括这四个组合中的每一个的谷氨酸传感器的1mM谷氨酸的阳极峰值电流。当NAD依赖性GDH沉积在rGO修饰的电极上时，谷氨酸响应最高。

更新日期：2018-08-22

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