High-throughput cell focusing and separation via acoustofluidic tweezers.,Lab on a Chip

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High-throughput cell focusing and separation via acoustofluidic tweezers.
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-08-22 00:00:00 , DOI: 10.1039/c8lc00434j
Mengxi Wu ₁ , Kejie Chen , Shujie Yang , Zeyu Wang , Po-Hsun Huang , John Mai , Zeng-Yao Li , Tony Jun Huang

Affiliation

Separation of particles and cells is an important function in many biological and biomedical protocols. Although a variety of microfluidic-based techniques have been developed so far, there is clearly still a demand for a precise, fast, and biocompatible method for separation of microparticles and cells. By combining acoustics and hydrodynamics, we have developed a method which we integrated into three-dimensional acoustofluidic tweezers (3D-AFT) to rapidly and efficiently separate microparticles and cells into multiple high-purity fractions. Compared with other acoustophoresis methods, this 3D-AFT method significantly increases the throughput by an order of magnitude, is label-free and gently handles the sorted cells. We demonstrate not only the separation of 10, 12, and 15 micron particles at a throughput up to 500 μl min⁻¹ using this 3D-AFT method, but also the separation of erythrocytes, leukocytes, and cancer cells. This 3D-AFT method is able to meet various separation demands thus offering a viable alternative with potential for clinical applications.

中文翻译：

高通量细胞聚焦和通过声流镊子分离。

在许多生物学和生物医学方案中，颗粒和细胞的分离是重要的功能。尽管到目前为止已经开发了多种基于微流体的技术，但是显然仍然需要用于分离微粒和细胞的精确，快速且生物相容的方法。通过将声学和流体动力学相结合，我们开发了一种方法，将其集成到三维声流体镊子（3D-AFT）中，可以快速有效地将微粒和细胞分离为多个高纯度级分。与其他声泳方法相比，此3D-AFT方法可将通量显着提高一个数量级，无标记，可轻柔地处理分选的细胞。我们不仅展示了以高达500μlmin的通量分离10、12和15微米颗粒的方法^-1使用此3D-AFT方法，但也分离红细胞，白细胞和癌细胞。这种3D-AFT方法能够满足各种分离需求，从而为临床应用提供了可行的替代方法。

更新日期：2018-08-22

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