The aim of this study was to investigate the cellular uptake and antitumor activity of 2 different structural flaxseed orbitides ([1–9-NαC]-linusorb B2 containing methionine residue and [1–9-NαC]-linusorb B3 containing isoleucine residue) in SGC-7901 cells as well as its underlying molecule mechanism. Results showed that the absorption efficiency of [1–9-NαC]-linusorb B3 was higher than that of [1–9-NαC]-linusorb B2. Besides, through the evaluation of mitochondrial membrane potential (Δφm), cytochrome c (Cyt C) release, caspase, death receptor activation and cleavage of PARP, we found that [1–9-NαC]-linusorb B3 exhibited more obvious apoptosis induction on SGC-7901 cells than [1–9-NαC]-linusorb B2. Interestingly, [1–9-NαC]-linusorb B3-induced cell apoptosis might involve a mitochondrial- and death receptor-mediated intrinsic and extrinsic pathway; however, [1–9-NαC]-linusorb B2 induced cell apoptosis by the activation of Fas, FasL, caspase 8 as well as caspase 3 instead of Bax, Cyt C, tBid and caspase 9, suggesting that extrinsic pathway might be involved in the apoptotic process.

这项研究的目的是研究两种不同结构的亚麻籽轨道蛋白（含有蛋氨酸残基的[1–9-NαC] -linusorb B2和含有异亮氨酸残基的[1–9-NαC] -linusorb B3）的细胞吸收和抗肿瘤活性。 SGC-7901细胞及其潜在的分子机制。结果表明，[1–9-NαC] -linusorb B3的吸收效率高于[1–9-NαC] -linusorb B2。此外，通过评估线粒体膜电位（Δφm），细胞色素c（Cyt C）释放，胱天蛋白酶，死亡受体激活和PARP裂解，我们发现[1–9-NαC] -linusorb B3在SGC-7901细胞上比[1–9-NαC] -linusorb B2表现出更明显的凋亡诱导作用。有趣的是，[1-9-NαC] -linusorb B3诱导的细胞凋亡可能涉及线粒体和死亡受体介导的内在和外在途径。然而，[1–9-NαC] -linusorb B2通过激活Fas，FasL，caspase 8以及caspase 3而不是Bax，Cyt C，tBid和caspase 9来诱导细胞凋亡，这表明外源性途径可能参与了该过程。凋亡过程。