Volatilization loss of analytes in open-vessel digestion is a concern for mercury speciation analysis. Loss of Hg⁰ vapour can be overcome by using oxidative digestion media, while loss of aerosols can be circumvented by complete condensation under sub-boiling-point heating. Using a simple system that consisted of a block heater and culture tubes, two digestion protocols were developed for determination of total Hg (tHg) and inorganic Hg (iHg). The resulting Hg⁺⁺ was selectively reduced by SnCl₂ to Hg⁰, which was detected by atomic fluorescence spectrometry (AFS). MeHg⁺ was oxidized to Hg⁺⁺ to yield tHg and calculated from the difference. These methods were validated using certified reference material (CRM) DORM-4, and applied to seafood analysis with 3.6 and 3.2 ng g⁻¹ (dry weight) limit of detection for iHg and tHg, respectively. Using only off-the-shelf components, these methods gained cost, simplicity, and parallel operation advantages.

开放容器消化中分析物的挥发损失是汞形态分析的关注点。Hg ⁰蒸气的损失可以通过使用氧化消化介质来克服，而气溶胶的损失可以通过在亚沸点加热下完全冷凝来避免。使用由块状加热器和培养管组成的简单系统，开发了两种消解方案，用于测定总Hg（tHg）和无机Hg（iHg）。通过SnCl ₂将生成的Hg ⁺⁺选择性还原为Hg ⁰，这通过原子荧光光谱法（AFS）进行检测。MeHg ⁺被氧化为Hg ⁺⁺产生tHg并根据差值计算。这些方法已通过认证参考材料（CRM）DORM-4进行了验证，并分别应用于海产品分析，其iHg和tHg的检出限分别为3.6和3.2 ng g ^-1（干重）。仅使用现成的组件，这些方法就获得了成本，简便性和并行操作优势。