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Optimization of in vitro carbohydrate digestion by mammalian mucosal α-glucosidases and its applications to hydrolyze the various sources of starches
Food Hydrocolloids ( IF 11.0 ) Pub Date : 2019-02-01 , DOI: 10.1016/j.foodhyd.2018.08.033
Hansol Shin , Dong-Ho Seo , Jungmin Seo , Lisa M. Lamothe , Sang-Ho Yoo , Byung-Hoo Lee

Abstract Glycemic carbohydrates, such as starch and sucrose, are fully hydrolyzed into monosaccharides by mucosal α-glucosidases in the small intestine. In this study, we optimized the reaction conditions of mammalian mucosal α-glucosidases to digest various types of glycemic carbohydrates to determine their hydrolytic activity. The results clearly showed that mammalian mucosal α-glucosidases had different hydrolytic properties compared to fungal amyloglucosidase, which is commonly used in vitro assay to determine carbohydrate quality, on various sizes and α-linked carbohydrates. Furthermore, non-gelatinized and gelatinized starches from different botanical sources were differently hydrolyzed to glucose by the prepared solution of mammalian α-glucosidases and porcine pancreatic α-amylase. Thus, it is clearly suggested that the digestion of glycemic carbohydrates by the optimized reaction condition with mammalian mucosal α-glucosidases and pancreatic α-amylase can be applied to determine the carbohydrate quality and digestion properties as it is more reliable to human digestive enzymes compared to fungal or microbial α-glucosidases.

中文翻译:

哺乳动物粘膜α-葡萄糖苷酶体外消化碳水化合物的优化及其在水解各种淀粉来源中的应用

摘要 糖类碳水化合物,如淀粉和蔗糖,在小肠内被黏膜α-葡萄糖苷酶完全水解成单糖。在这项研究中,我们优化了哺乳动物粘膜α-葡萄糖苷酶的反应条件,以消化各种类型的血糖碳水化合物,以确定它们的水解活性。结果清楚地表明,与真菌淀粉葡萄糖苷酶相比,哺乳动物粘膜 α-葡萄糖苷酶具有不同的水解特性,真菌淀粉葡萄糖苷酶通常用于体外测定以确定碳水化合物质量,对各种大小和 α-连接的碳水化合物。此外,来自不同植物来源的非糊化和糊化淀粉被哺乳动物α-葡萄糖苷酶和猪胰腺α-淀粉酶的制备溶液不同地水解为葡萄糖。因此,
更新日期:2019-02-01
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