Quorum Sensing Can Be Repurposed To Promote Information Transfer between Bacteria in the Mammalian Gut,ACS Synthetic Biology

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Quorum Sensing Can Be Repurposed To Promote Information Transfer between Bacteria in the Mammalian Gut
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2018-08-20 00:00:00 , DOI: 10.1021/acssynbio.8b00271
Suhyun Kim _{1,

2,

3} , S. Jordan Kerns _{1,

3} , Marika Ziesack _{1,

3} , Lynn Bry ₄ , Georg K. Gerber ₄ , Jeffrey C. Way _{1,

3} , Pamela A. Silver _{1,

3}

Affiliation

The gut microbiome is intricately involved with establishing and maintaining the health of the host. Engineering of gut microbes aims to add new functions and expand the scope of control over the gut microbiome. To create systems that can perform increasingly complex tasks in the gut, it is necessary to harness the ability of the bacteria to communicate in the gut environment. Interestingly, acyl-homoserine lactone (acyl-HSL)-mediated Gram-negative bacterial quorum sensing, a widely used mode of intercellular signaling system in nature, has not been identified in normal healthy mammalian gut. It remains unknown whether the gut bacteria that do not natively use quorum sensing can be engineered to successfully signal to other bacteria using acyl-HSLs in the gut environment. Here, we repurposed quorum sensing to create an information transfer system between native gut Escherichia coli and attenuated Salmonella enterica serovar Typhimurium. Specifically, we functionalized one species with inducible signal production and the other with signal detection and recording using genomically integrated circuits. The information transfer system demonstrated successful intra- and interspecies signaling in the murine gut. This study provides a basis for further understanding of interbacterial interactions in an otherwise hard-to-study environment as well as a basis for further investigation of the potential of acyl-HSLs as intercellular signaling molecules of engineered gut consortia.

中文翻译：

可以将群体感应重新用于促进哺乳动物肠道细菌之间的信息传递。

肠道微生物组与建立和维持宿主的健康息息相关。肠道微生物工程旨在增加新功能并扩大对肠道微生物组的控制范围。为了创建可以在肠道中执行日益复杂的任务的系统，必须利用细菌在肠道环境中进行交流的能力。有趣的是，在正常健康的哺乳动物肠道中，尚未鉴定出酰基-高丝氨酸内酯（酰基-HSL）介导的革兰氏阴性细菌群体感应，这是自然界中广泛使用的细胞间信号系统的模式。尚不知道是否可以将天然不使用群体感应的肠道细菌改造成能够在肠道环境中使用酰基-HSL成功向其他细菌发出信号。这里，大肠杆菌和减毒肠炎沙门氏菌鼠伤寒沙门氏菌。具体来说，我们使用诱导型信号产生功能化了一个物种，而使用基因组集成电路对另一个物种进行了信号检测和记录。信息传递系统证明了鼠肠道内种内和种间成功的信号传递。这项研究为进一步理解在其他情况下难以研究的环境中的细菌相互作用提供了基础，也为进一步研究酰基-HSL作为工程肠道菌群的细胞间信号分子的潜力提供了基础。

更新日期：2018-08-20

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