Although current implementations of super-resolution microscopy are technically approaching true molecular-scale resolution, this has not translated to imaging of biological specimens, because of the large size of conventional affinity reagents. Here we introduce slow off-rate modified aptamers (SOMAmers) as small and specific labeling reagents for use with DNA points accumulation in nanoscale topography (DNA-PAINT). To demonstrate the achievable resolution, specificity, and multiplexing capability of SOMAmers, we labeled and imaged both transmembrane and intracellular targets in fixed and live cells.

中文翻译：

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修饰适配体可实现亚 10 nm 细胞 DNA-PAINT 定量成像

尽管目前超分辨率显微镜的实现在技术上已接近真正的分子尺度分辨率，但由于传统亲和试剂的尺寸较大，这尚未转化为生物样本的成像。在这里，我们引入慢解离速率修饰适体 (SOMAmers) 作为小型特异性标记试剂，用于纳米级形貌 (DNA-PAINT) 中 DNA 点积累。为了证明 SOMAmers 可实现的分辨率、特异性和多重功能，我们对固定细胞和活细胞中的跨膜和细胞内靶标进行了标记和成像。