An assay protocol, based on programmable Flow Injection (pFI), is optimized by tailoring flowrates appropriately to the individual steps of an assay, thus allowing sample and reagent metering, mixing, incubation, monitoring and washout to be carried out more efficiently and in different time frames. This novel approach to flow based methods is applied here to optimize the determination of orthophosphate at nanomolar levels. Programmable Flow Injection was also used to facilitate an investigation of the properties of the phosphomolybdenum blue (PMoB) formed during this assay, by using the stop flow technique – an approach that revealed for the first time the influence of surfactants on the kinetics of formation of PMoB and its spectral characteristics. It was discovered that the two most frequently used surfactants (SDS and Brij) have profound and different influences on the spectra and formation of PMoB and this finding was used to enhance the sensitivity of the phosphate assay at nanomolar levels. The method was applied to the assay of trace levels of phosphate in sea water.

通过根据实验的各个步骤适当调整流速，优化了基于可编程流动注射（pFI）的实验方案，从而使样品和试剂的计量，混合，温育，监测和洗脱得以更有效且以不同方式进行时间范围。这种基于流动的方法的新颖方法在此适用于以纳摩尔水平优化正磷酸盐的测定。可编程流动注射还用于通过使用停止流动技术促进对该试验过程中形成的磷钼蓝（PMoB）性质的研究，该方法首次揭示了表面活性剂对表面活性剂形成的动力学的影响。 PMoB及其光谱特性。已发现两种最常用的表面活性剂（SDS和Brij）对PMoB的光谱和形成有深刻而不同的影响，这一发现可用于增强纳摩尔水平的磷酸盐测定的灵敏度。该方法适用于海水中痕量磷酸盐的测定。