Few studies have evaluated the anti-digestion effects of grape seed procyanidins (GSPs) compared to their strong anti-oxidant activities according to different degrees of polymerization (DPs). Additionally, the effects of GSPs with different DPs on the binding sites of digestive enzymes are unexplored. In this work, the anti-digestive and anti-oxidant activity of GSPs with different DPs were verified and compared. Anti-digestive activity assays were conducted on key digestive enzymes. Although all procyanidins fractions possessed antioxidant activity as expected, there was no significant difference in the antioxidant activity among procyanidins with different DPs. In contrast, the anti-digestive activity of the fractions increased significantly as the DP increased. Meanwhile, molecular docking provided a putative mechanism of anti-digestion, and GSPs can block the enzyme sites by hydrogen interactions, hydrophobic interactions and electrostatic interactions. These results indicated that the higher-molecular-weight procyanidins had equal or lower antioxidant activity and a greater anti-digestion effect than lower-molecular-weight procyanidins.

很少有研究评估葡萄籽原花青素（GSP）的抗消化作用，以及根据不同聚合度（DPs）的强抗氧化活性。另外，尚未探索具有不同DP的GSP对消化酶结合位点的影响。在这项工作中，验证并比较了具有不同DP的GSP的抗消化和抗氧化活性。对关键的消化酶进行了抗消化活性测定。尽管所有原花青素级分均具有预期的抗氧化活性，但在具有不同DP的原花青素中，其抗氧化活性没有显着差异。相反，级分的抗消化活性随着DP的增加而显着增加。同时，分子对接提供了一种抗消化的推定机制，GSP可以通过氢相互作用，疏水相互作用和静电相互作用来阻断酶的位点。这些结果表明，与较低分子量的原花青素相比，较高分子量的原花青素具有相同或较低的抗氧化活性，并且具有更好的抗消化作用。