This study investigated the effect of xanthohumol (XN), a flavonoid present in hops (Humulus lupulus L.), against ageing-related heart alterations in male senescence-accelerated prone mice (SAMP8). Male senescence-accelerated resistant mice (SAMR1) were used as controls. Animals were divided into four experimental groups of each strain: non-treated young mice, non-treated old mice and old mice treated either with 1 mg/kg/day or 5 mg/kg/day XN. After 30 days of treatment, animals were sacrificed and their hearts were collected and immediately frozen. mRNA and protein expressions of TNF-α, IL-1β, NFκB p65, HO-1, HO-2, BAX, caspase 3, AIF and SIRT1 were measured by RT-PCR and Western blot, respectively. Our results showed an increase in inflammatory, oxidative stress and apoptosis markers in heart of old SAMP8 mice, in contrast to their younger counterparts. After treatment with XN, these age-related changes were reversed suggesting that XN could prevent the appearance of cardiovascular alterations related to ageing.

本研究调查黄腐酚（XN），类黄酮存在于啤酒花（效果啤酒花L.）对抗雄性衰老加速易感小鼠（SAMP8）中与衰老相关的心脏改变。将雄性衰老加速抗性小鼠（SAMR1）用作对照。将动物分成每种菌株的四个实验组：未治疗的年轻小鼠，未治疗的老小鼠和用1mg / kg /天或5mg / kg /天XN处理的老小鼠。治疗30天后，处死动物，收集心脏并立即冷冻。分别通过RT-PCR和Western blot检测TNF-α，IL-1β，NFκBp65，HO-1，HO-2，BAX，caspase 3，AIF和SIRT1的mRNA和蛋白表达。我们的研究结果表明，与年轻的SAMP8小鼠相比，SAMP8小鼠的心脏中炎症，氧化应激和凋亡标记物增加。用XN治疗后，