Six undescribed withanolides were isolated and characterized during the investigation of anti-inflammatory and cytotoxic constituents from the whole plants of Physalis minima L. Their structures were elucidated by extensive spectroscopic analyses (IR, UV, HR-ESI-MS, 1D-NMR, and 2D-NMR), and their anti-inflammatory and cytotoxic activities were evaluated in vitro. All the compounds exhibited anti-inflammatory ability via inhibiting the production of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cells. Moderate cytotoxic activities against A549 lung adenocarcinoma cells, SMMC-7721 hepatic carcinoma cells and MCF-7 breast cancer cells with IC50 values in the range of 40.01-82.17 μM were observed for these withanolides.

中文翻译：

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来自 Physalis minima 的抗炎和细胞毒性 withanolides

在研究 Physalis minima L 全株的抗炎和细胞毒性成分期间，分离和表征了六种未描述的 withanolides。它们的结构通过广泛的光谱分析（IR、UV、HR-ESI-MS、1D-NMR 和2D-NMR），并在体外评估了它们的抗炎和细胞毒性活性。所有化合物均通过抑制脂多糖 (LPS) 刺激的小鼠巨噬细胞 RAW 264.7 细胞中一氧化氮 (NO) 的产生而表现出抗炎能力。观察到对 A549 肺腺癌细胞、SMMC-7721 肝癌细胞和 MCF-7 乳腺癌细胞具有中等细胞毒活性，这些 withanolides 的 IC50 值在 40.01-82.17 μM 范围内。