In this study, the impacts of heavy metals (1 mg L⁻¹) on the nitrogen removal, bioactivity of anaerobic ammonia-oxidizing bacteria (AAOB) and the microbial community of anaerobic ammonium oxidation (Anammox) process were investigated. It was observed that short-term exposure in Cu (II) and Zn (II) both improved AAOB bioactivity, while long-term exposure significantly lowered the nitrogen removal to 0.218 and 0.302 kg m⁻³ d⁻¹, when treated the wastewater with 100 mg L⁻¹ nitrogen under 14–16 °C. Fe(II) had slight impact on Anammox in short-term experiment but deeply enhanced nitrogen removal during the long-term contact, and finally increased the that to 0.58 kg m⁻³ d⁻¹. The impact on Anammox was Cu(II) > Zn(II) > Fe(II). Cu(II) and Zn(II) lowered the share of Candidatus Kuenenia to 3.32% and 3.80%, while Fe(II) improved that to 11.30% from 7.99%. Extracellular polymeric substance in biofilm had prominent iron adsorption capacity, which was the key factor that help AAOB resist Fe(II).

在这项研究中，研究了重金属（1 mg L ^-1 ）对脱氮，厌氧氨氧化细菌（AAOB）的生物活性以及厌氧铵氧化（Anammox）过程中微生物群落的影响。观察到，当用废水处理废水时，Cu（II）和Zn（II）中的短期暴露均改善了AAOB的生物活性，而长期暴露则将氮的去除量显着降低至0.218和0.302 kg m ^-3  d ^-1 。在14–16°C下100 mg L ^-1氮。Fe（II）在短期实验中对Anammox的影响很小，但在长期接触过程中显着增强了氮的去除，最终将其增加至0.58 kg m ^-3  d ^-1。对厌氧氨氧化的影响是Cu（II）> Zn（II）> Fe（II）。Cu（II）和Zn（II）将Candidatus Kuenenia的份额降低到3.32％和3.80％，而Fe（II）的份额从7.99％提高到11.30％。生物膜中的细胞外聚合物质具有突出的铁吸附能力，这是帮助AAOB抵抗Fe（II）的关键因素。