Stroke causes an efflux of various groups of progenitor/stem cells from bone marrow to bloodstream and a rise in neuron specific enolase (NSE) serum concentrations. The aim of this study was to identify activity of chosen stem/progenitor cells during first 7 days after stroke through correlations between these cells levels and NSE values. Additional goal was to confirm the role of NSE as a prognostic marker of ischemic stroke. Venous blood was collected repeatedly from 67 acute ischemic stroke patients and 15 control subjects, in order to assess NSE with ELISA, and CD45⁻CD34 ⁺ CD271+, CD45⁻CD34 + CXCR4+, CD45⁻CD34 + CXCR7+ and CD45⁻CD34 ⁺ CD133 + stem/progenitor cells by means of flow cytometry. Patients underwent repeated assessment with the National Ischemic Stroke Scale and modified Rankin Scale. Ischemic lesion volumes were assessed twice by MRI-DWI (day 1 and 5 ± 2). NSE correlated negatively with MFI levels of the CD45⁻CD34 + CXCR7+ cells, and percentage levels of the CD45⁻CD34 ⁺ and CD45⁻CD34 + CXCR4+ cells. NSE concentrations were significantly higher in patients compared to control subjects. NSE on day 2 positively correlated with lesion volume on both MRI. NSE on day 2 and 6–7 correlated positively with initial NIHSS scores, and on day 1 with mRS score on day 9.

In conclusion, in this study NSE indicated some activity of the CD45⁻CD34 + CXCR7+, CD45⁻CD34 ⁺ and CD45⁻CD34 + CXCR4+ stem/progenitor cells in the first 7 days after ischemic stroke. Additionally, this study supports the thesis that NSE might be a valuable prognostic marker in acute ischemic stroke.

中风引起从骨髓到血流的各种祖细胞/干细胞外流，并且神经元特异性烯醇化酶（NSE）血清浓度升高。这项研究的目的是通过卒中后的前7天中这些细胞水平与NSE值之间的相关性来鉴定所选干/祖细胞的活性。另一个目标是确认NSE作为缺血性中风的预后标志物的作用。为了通过ELISA评估NSE，以及从CD45 ^- CD34  ⁺  CD271 +，CD45 ^- CD34 + CXCR4 +，CD45 ^- CD34 + CXCR7 +和CD45 ^- CD34  ^+，从67名急性缺血性中风患者和15名对照受试者中反复收集静脉血。 通过流式细胞术检测CD133 +干/祖细胞。使用美国国家缺血性卒中量表和改良的兰金量表对患者进行反复评估。MRI-DWI对缺血性病变体积进行了两次评估（第1天和第5±2天）。NSE与CD45 ^- CD34 + CXCR7 +细胞的MFI水平以及CD45 ^- CD34  ⁺ 和CD45 ^- CD34 + CXCR4 +细胞的百分比水平呈负相关。与对照组相比，患者的NSE浓度明显更高。第2天的NSE与两个MRI上的病变体积均呈正相关。第2天和第6-7天的NSE与初始NIHSS得分呈正相关，而第1天的NSE与第9天的mRS得分呈正相关。

总之，在这项研究中，NSE提示缺血性卒中后的前7天CD45 ^- CD34 + CXCR7 +，CD45 ^- CD34  ⁺ 和CD45 ^- CD34 + CXCR4 +干/祖细胞有一定活性。此外，本研究支持以下观点：NSE可能是急性缺血性卒中的有价值的预后标志物。