We investigated the effect of a lemon-peel extract in a rat model of rheumatoid arthritis and SW1353 chondrocytes. The rats were divided into control (sham), rheumatoid arthritis (control), extract (150 mg/kg bwt) and extract (300 mg/kg bwt) groups. Pyrogallic acid, caffeic acid, eugenol, p-coumaric acid, p‑hydroxyl benzoic acid, resorcinol, salicylic acid, luteoline, quercetin, chrysin, and luteolin-3‑methoxy‑7-rutinoside were noted in the extracts. The extract was administered orally for five consecutive weeks, and antioxidant and inflammatory markers were investigated. Extracts markedly increased the plasma levels of catalase, superoxide dismutase, reduced glutathione, glutathione peroxidase and zinc, and reduced those of lipid peroxidation, nitric oxide, uric acid, ceruloplasmin, copper, matrix metalloproteinase-3 and prostaglandin E₂. Moreover, the extracts reduced the serum levels of interleukin-1 beta, tumor necrosis factor-alpha and interleukin-6. Extracts at 50, 100 and 150 mg/L reduced chondrocyte proliferation by 20.9, 38.8, and 52.1%, respectively, and reduced the reactive oxygen species and NF-κB levels by > 40%. Furthermore, the lemon-peel extract at 50, 100 and 150 mg/L reduced the xanthine oxidase level by 0.26-, 0.55- and 0.89-fold, respectively. Taking all these data together, it is suggested the lemon peel extract ameliorated rheumatoid arthritis, reduced the SW1353 chondrocyte proliferation, ROS, inflammatory cytokines, and xanthine oxidase levels.

我们调查了柠檬皮提取物在类风湿性关节炎和SW1353软骨细胞大鼠模型中的作用。将大鼠分为对照组（假），类风湿性关节炎（对照组），提取物（150mg / kg bwt）和提取物（300mg / kg bwt）组。提取物中发现了邻苯二酸，咖啡酸，丁子香酚，对香豆酸，对羟基苯甲酸，间苯二酚，水杨酸，黄体碱，槲皮素，黄体素和木犀草素-3-甲氧基-7-芸香糖苷。提取物连续五周口服给药，并研究了抗氧化剂和炎性标志物。提取物可显着提高血浆过氧化氢酶，超氧化物歧化酶，减少谷胱甘肽，谷胱甘肽过氧化物酶和锌的含量，并降低脂质过氧化，一氧化氮，尿酸，铜蓝蛋白，铜，基质金属蛋白酶-3和前列腺素E的水平。₂。此外，提取物降低了血清白介素-1β，肿瘤坏死因子-α和白介素-6的血清水平。50、100和150 mg / L的提取物分别将软骨细胞的增殖降低了20.9％，38.8％和52.1％，并将活性氧和NF-κB水平降低了40％以上。此外，柠檬皮提取物的浓度分别为50、100和150 mg / L，使黄嘌呤氧化酶水平分别降低了0.26、0.55和0.89倍。综合所有这些数据，建议柠檬皮提取物改善类风湿性关节炎，减少SW1353软骨细胞增殖，ROS，炎性细胞因子和黄嘌呤氧化酶水平。