A variant of the hybridization oligonucleotide microarray, utilizing the principle of many probes-one spot (MPOS-microarrays), is proposed. A case study based on Orthopoxviruses (Variola, Monkeypox, and Ectromelia viruses) demonstrates a considerable increase in the fluorescence signal (up to 100-fold) when several oligonucleotide probes are printed to one spot. Moreover, the specificity of detection also increases (almost 1000-fold), allowing the use of probes that individually lack such high specificity. The optimal probes have a Tm of 32–37 °C and length of 13–15 bases. We suggest that the high specificity and sensitivity of the MPOS-microarray is a result of cooperativity of DNA binding with all probes immobilized in the spot. This variant of DNA detection can be useful for designing biosensors, tools for point-of-care (POC) diagnostics, microbial ecology, analysis of clustered regularly interspaced short palindromic repeats (CRISPR), and others.

Open image in new window

提出了杂交寡核苷酸微阵列的一种变体，它利用了许多探针一点的原理（MPOS-微阵列）。基于为例正痘病毒（天花，猴痘和鼠痘病毒）展示了当几个寡核苷酸探针被印到一个点时荧光信号的显着增加（最多100倍）。此外，检测的特异性也增加了（几乎是1000倍），从而允许使用个别缺乏这种高特异性的探针。最佳探针的Tm为32–37°C，长度为13–15个碱基。我们认为，MPOS-微阵列的高特异性和敏感性是DNA与固定在该点上的所有探针结合的协同作用的结果。DNA检测的这种变体可用于设计生物传感器，即时诊断（POC）诊断工具，微生物生态学，聚类的规则间隔的短回文重复序列（CRISPR）等。

在新窗口中打开图像