Identification of Missing Proteins in Human Olfactory Epithelial Tissue by Liquid Chromatography–Tandem Mass Spectrometry,Journal of Proteome Research

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Identification of Missing Proteins in Human Olfactory Epithelial Tissue by Liquid Chromatography–Tandem Mass Spectrometry
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-08-20 , DOI: 10.1021/acs.jproteome.8b00408
Heeyoun Hwang _{1,

2,

3} , Ji Eun Jeong _{1,

2} , Hyun Kyoung Lee _{1,

2} , Ki Na Yun _{1,

4} , Hyun Joo An _{2,

3} , Bonghee Lee _{5,

6} , Young-Ki Paik ₇ , Tae Seok Jeong ₈ , Gi Taek Yee ₈ , Jin Young Kim ₁ , Jong Shin Yoo _{1,

2}

Affiliation

We performed proteomic analyses of human olfactory epithelial tissue to identify missing proteins using liquid chromatography–tandem mass spectrometry. Using a next-generation proteomic pipeline with a < 1.0% false discovery rate at the peptide and protein levels, we identified 3731 proteins, among which five were missing proteins (P0C7M7, P46721, P59826, Q658L1, and Q8N434). We validated the identified missing proteins using the corresponding synthetic peptides. No olfactory receptor (OR) proteins were detected in olfactory tissue, suggesting that detection of ORs would be very difficult. We also identified 49 and 50 alternative splicing variants mapped at the neXtProt and GENCODE databases, respectively, and 2000 additional single amino acid variants. This data set is available at the ProteomeXchange consortium via PRIDE repository (PXD010025).

中文翻译：

液相色谱-串联质谱法鉴定人嗅上皮组织中缺失的蛋白质

我们使用液相色谱-串联质谱法对人的嗅觉上皮组织进行了蛋白质组学分析，以鉴定缺失的蛋白质。使用在肽和蛋白质水平上错误发现率小于1.0％的下一代蛋白质组学流水线，我们鉴定了3731种蛋白质，其中5种缺失蛋白质（P0C7M7，P46721，P59826，Q658L1和Q8N434）。我们使用相应的合成肽验证了鉴定出的缺失蛋白。在嗅觉组织中未检测到嗅觉受体（OR）蛋白，这表明ORs的检测非常困难。我们还确定了分别映射到neXtProt和GENCODE数据库的49和50个替代剪接变体，以及2000个其他单氨基酸变体。

更新日期：2018-11-29

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