The present study evaluated the neuroprotective effects of one selenium-containing AZT derivative compound (S1073) in memory and learning impairment caused by Intracerebroventricular-streptozotocin (ICV-STZ). ICV-STZ in mice causes impairment of energy metabolism with oxidative damage and cholinergic dysfunction, and provides a relevant model for sporadic dementia of Alzheimer's type (AD). Acetylcolinesterase (AChE), Catalase (CAT), dichlorofluorescein oxidation (DCFH), TBARS and thiol content were measured. Swiss adult mice were pre-treated with S1073 [1 mmol/kg] (i.p.) and after 30 min of the injection received a bilateral dose of STZ [11.3 μmol/l]. After 8 days' STZ injection, we performed the behavioral experiments (Beaker test, Open field and Morris water maze task). ICV-STZ caused significant learning and memory impairments, which were significantly improved by S1073 pre-treatment. A significant increase in cerebral DFCH, TBARS levels and AChE activity and a disturbance in the memory and learning were observed in ICV-STZ injected animals. S1073 significantly ameliorated all alterations induced by ICV-STZ in mice. All these findings support the neuroprotective role of S1073 in mice model of Alzheimer's dementia-type induced by ICV-STZ, which may be associated with its antioxidant activity and/or with its inhibitory effect in brain AChE. In fact, in silico analysis indicated that S1073 may be an inhibitor of AChE.

本研究评估了一种含硒的AZT衍生物化合物（S1073）在对脑室内室链脲佐菌素（ICV-STZ）引起的记忆和学习障碍中的神经保护作用。小鼠中的ICV-STZ导致能量代谢受损，并伴有氧化损伤和胆碱能功能障碍，并为阿尔茨海默氏病（AD）偶发性痴呆症提供了相关模型。测量了乙酰胆碱酯酶（AChE），过氧化氢酶（CAT），二氯荧光素氧化（DCFH），TBARS和硫醇含量。瑞士成年小鼠用S1073 [1 mmol / kg]（ip）进行了预处理，注射30分钟后接受了STZ的双边剂量[11.3μmol/ l]。注射STZ 8天后，我们进行了行为实验（烧杯测试，开阔野外和莫里斯水迷宫任务）。ICV-STZ导致严重的学习和记忆障碍，通过S1073预处理可以显着改善。在注射ICV-STZ的动物中观察到脑DFCH，TBARS水平和AChE活性显着增加，并且记忆和学习受到干扰。S1073显着改善了ICV-STZ诱导的小鼠所有改变。所有这些发现支持S1073在ICV-STZ诱导的阿尔茨海默氏痴呆型小鼠模型中的神经保护作用，这可能与其抗氧化活性和/或其对脑AChE的抑制作用有关。实际上，所有这些发现支持S1073在ICV-STZ诱导的阿尔茨海默氏痴呆型小鼠模型中的神经保护作用，这可能与其抗氧化活性和/或其对脑AChE的抑制作用有关。实际上，所有这些发现都支持S1073在ICV-STZ诱导的阿尔茨海默氏痴呆型小鼠模型中的神经保护作用，这可能与其抗氧化活性和/或其对脑AChE的抑制作用有关。实际上，计算机分析的结果表明S1073可能是AChE的抑制剂。