Reversible Supramolecular Assembly of Velvet Worm Adhesive Fibers via Electrostatic Interactions of Charged Phosphoproteins,Biomacromolecules

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Reversible Supramolecular Assembly of Velvet Worm Adhesive Fibers via Electrostatic Interactions of Charged Phosphoproteins
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-08-16 00:00:00 , DOI: 10.1021/acs.biomac.8b01017
Alexander Baer ₁ , Sebastian Hänsch ₂ , Georg Mayer ₁ , Matthew J. Harrington _{3,

4} , Stephan Schmidt ₅

Affiliation

Velvet worms secrete a fluid hunting slime comprised of a dispersion of nanoglobules that form microfibers under small mechanical shear forces, facilitating the rapid formation of stiff biopolymeric fibers. Here, we demonstrate that the nanoglobules are held together and stabilized as a dispersion by electrostatic interactions reminiscent of coacervate-based natural adhesives. Variation of ionic strength and pH affects the stability of nanoglobules and their ability to form fibers. Fibers mainly consist of large (∼300 kDa), highly charged proteins, and current biochemical analysis reveals a high degree of protein phosphorylation and presence of divalent cations. Taken together, we surmise that polyampholytic protein sequences, phosphorylated sites, and ions give rise to transient ionic cross-linking, enabling reversible curing of ejected slime into high-stiffness fibers following dehydration. These results provide a deeper understanding of velvet worm adhesive fibers, which may stimulate new routes toward mechanoresponsive and sustainable materials.

中文翻译：

通过带电的磷酸蛋白的静电相互作用的天鹅绒蠕虫粘合纤维的可逆超分子组装。

天鹅绒蠕虫分泌一种由纳米球的分散体组成的液体狩猎粘液，该分散体在较小的机械剪切力作用下形成微纤维，有助于快速形成坚硬的生物聚合物纤维。在这里，我们证明了纳米球保持在一起并通过静电相互作用使稳定性稳定为分散体，而这种相互作用使人联想到凝聚层为基础的天然胶粘剂。离子强度和pH的变化会影响纳米球的稳定性及其形成纤维的能力。纤维主要由大（〜300 kDa），带高电荷的蛋白质组成，目前的生化分析表明，蛋白质的磷酸化程度很高，并且存在二价阳离子。综上所述，我们推测多溶性蛋白序列，磷酸化位点和离子会引起短暂的离子交联，脱水后可将喷射出的粘液可逆固化成高硬度纤维。这些结果提供了对天鹅绒蠕虫粘合纤维的更深入的了解，这可能会激发出新的机械响应性和可持续性材料的途径。

更新日期：2018-08-16

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