Simultaneous analyses of peripheral and mucosal immune compartments can yield insight into the pathogenesis of mucosal-associated diseases. Although methods to preserve peripheral immune cells are well established, studies involving mucosal immune cells have been hampered by lack of simple storage techniques. We provide a cryopreservation protocol allowing for storage of gastrointestinal (GI) tissue with preservation of viability and functionality of both immune and epithelial cells. These methods will facilitate translational studies allowing for batch analysis of mucosal tissue to investigate disease pathogenesis, biomarker discovery and treatment responsiveness.

中文翻译：

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高维免疫表型分析和转录分析表明，从冷冻的胃肠道组织中可以稳健地恢复有活力的人体免疫细胞和上皮细胞。

同时分析外周和粘膜免疫区室可以深入了解粘膜相关疾病的发病机制。尽管保存外周免疫细胞的方法已经很成熟，但由于缺乏简单的储存技术，涉及粘膜免疫细胞的研究受到阻碍。我们提供冷冻保存方案，允许储存胃肠道 (GI) 组织，同时保留免疫细胞和上皮细胞的活力和功能。这些方法将促进转化研究，允许对粘膜组织进行批量分析，以研究疾病发病机制、生物标志物发现和治疗反应。