The implementation of a numerical dark field illumination to obtain reconstructions with enhanced contrast in digital lensless holographic (DLHM) microscopy is presented. In this proposal, an opaque circular stop is numerically inserted in the light path between the sample and hologram plane. The contrast of the reconstructed holograms is increased at least 46% in comparison with that obtained by the regular reconstruction methods. This proposal has been validated using experimental holograms of a paramecium in water and a section of the head of a Drosophila melanogaster fly recorded in DLHM.

中文翻译：

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数值暗场照明应用于实验性数字无透镜全息显微镜，以增强对比度

提出了一种数字暗场照明的实现方式，以在数字无透镜全息（DLHM）显微镜中获得具有增强对比度的重建。在该建议中，将不透明的圆形光阑数值插入样品和全息图平面之间的光路中。与常规重建方法相比，重建全息图的对比度提高了至少46％。该提议已使用DLHM中记录的水中草履虫和黑腹果蝇蝇头头部的实验全息图进行了验证。