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Optofluidic differential colorimetry for rapid nitrite determination†
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-08-14 00:00:00 , DOI: 10.1039/c8lc00690c
Y. Shi 1, 2, 3, 4, 5 , H. L. Liu 1, 2, 3, 4, 5 , X. Q. Zhu 1, 2, 3, 4, 5 , J. M. Zhu 1, 2, 3, 4, 5 , Y. F. Zuo 1, 2, 3, 4, 5 , Y. Yang 1, 2, 3, 4, 5 , F. H. Jiang 5, 6, 7 , C. J. Sun 5, 6, 7 , W. H. Zhao 5, 8, 9 , X. T. Han 5, 8, 9
Affiliation  

Nitrite detection plays a very important role in environmental monitoring and for industrial purposes. The commonly used colorimetric analysis requires the measurement of a system's calibration curve by asynchronously preparing and detecting a dozen standard samples, leading to time-consuming, slow and cumbersome procedures. Here, we present a differential colorimetry method that determines the nitrite level based on the paired chromaticity gradient, formed by coupling the colour reaction into the microfluidic network. The two gradients reshape each other and contain enough information for the quantitative analysis of the sample being tested, without the need for a calibration curve. The independence of the two gradients of the absorbance change caused by the detecting system and water quality results in a high stability and anti-interference performance, with the assistance of its self-correcting ability. This differential colorimetry method requires little time and energy consumption as only one sample is needed. Standard nitrite solutions of 0.50 mM and 0.33 mM have been determined with an error of 1.16% and 0.50%, respectively. These measurements are advantageous in terms of greater stability by up to 10 times and accuracy by 6 times, compared with the calibration curve approaches. It is foreseeable that this differential colorimetry method will find a wide range of applications in the field of chemical detection.

中文翻译:

光流体微分比色法快速测定亚硝酸盐

亚硝酸盐检测在环境监测和工业用途中起着非常重要的作用。常用的比色分析需要通过异步准备和检测十二个标准样品来测量系统的校准曲线,从而导致耗时,缓慢且繁琐的过程。在这里,我们提出了一种差分比色法,该方法基于成对的色度梯度确定亚硝酸盐含量,该成对的色度梯度是通过将显色反应耦合到微流体网络中而形成的。这两个梯度相互重塑,并包含足够的信息,可以对被测样品进行定量分析,而无需校准曲线。由检测系统和水质引起的两个吸光度变化梯度的独立性,借助其自校正能力,可实现高稳定性和抗干扰性能。这种微分比色法只需要一个样品就需要很少的时间和能量消耗。已确定0.50 mM和0.33 mM的标准亚硝酸盐溶液的误差分别为1.16%和0.50%。与校准曲线方法相比,这些测量的优势在于其稳定性最高可达10倍,而精度则高达6倍。可以预见,这种差分比色法将在化学检测领域中找到广泛的应用。这种微分比色法只需要一个样品就需要很少的时间和能量消耗。已测定0.50 mM和0.33 mM的标准亚硝酸盐溶液,其误差分别为1.16%和0.50%。与校准曲线方法相比,这些测量的优势在于其稳定性最高可达10倍,而精度则高达6倍。可以预见,这种差分比色法将在化学检测领域中找到广泛的应用。这种微分比色法只需要一个样品就需要很少的时间和能量消耗。已确定0.50 mM和0.33 mM的标准亚硝酸盐溶液的误差分别为1.16%和0.50%。与校准曲线方法相比,这些测量的优势在于其稳定性最高可达10倍,而精度则高达6倍。可以预见,这种差分比色法将在化学检测领域中找到广泛的应用。与校准曲线方法比较。可以预见,这种差分比色法将在化学检测领域中找到广泛的应用。与校准曲线方法比较。可以预见,这种差分比色法将在化学检测领域中找到广泛的应用。
更新日期:2018-08-14
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