Tailor-made Janus lectin with dual avidity assembles glycoconjugate multilayers and crosslinks protocells†,Chemical Science

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Tailor-made Janus lectin with dual avidity assembles glycoconjugate multilayers and crosslinks protocells†
Chemical Science ( IF 7.6 ) Pub Date : 2018-08-14 00:00:00 , DOI: 10.1039/c8sc02730g
João P. Ribeiro _{1,

2,

3,

4,

5} , Sarah Villringer _{6,

7,

8,

9,

10} , David Goyard _{1,

2,

4,

5,

11} , Liliane Coche-Guerente _{1,

2,

4,

5,

11} , Manuela Höferlin _{6,

7,

8,

9,

10} , Olivier Renaudet _{1,

2,

4,

5,

11} , Winfried Römer _{6,

7,

8,

9,

10} , Anne Imberty _{1,

2,

3,

4,

5}

Affiliation

We engineered the first chimeric, bispecific lectin, with two rationally oriented and distinct recognition surfaces. This lectin, coined Janus lectin in allusion to the two-faced roman god, is able to bind independently to both fucosylated and sialylated glycoconjugates. The multivalent presentation of binding sites on each face of the Janus lectin is very efficient, resulting in avidities in the low nanomolar range for both fucosylated and sialylated surfaces. Moreover, novel heterovalent, bifunctional glycoclusters were synthetized that match the topology of the Janus lectin. Based on these tools, we constructed organized and controlled supramolecular architectures by assembling Janus lectin and glycocompound layer-by-layer. Furthermore, the Janus lectin was employed as biomolecular linker to organize protocells made from giant unilamellar vesicles of different nature, to more complex prototissues. In summary, tailor-made Janus lectins open wide possibilities for creating biomimetic matrices or artificial tissues.

中文翻译：

量身定制的具有双重亲和力的Janus凝集素可组装糖缀合物多层并交联原始细胞†

我们设计了第一个嵌合的，双特异性的凝集素，具有两个方向合理且截然不同的识别表面。这种凝集素是在两面罗马神的寓言中创造的贾纳斯凝集素，能够独立地与岩藻糖基化和唾液酸化的糖缀合物结合。Janus凝集素各面上结合位点的多价呈递非常有效，对于岩藻糖基化和唾液酸化的表面，亲合力都在低纳摩尔范围内。而且，合成了与Janus凝集素的拓扑结构相匹配的新颖的异价双功能糖簇。基于这些工具，我们通过逐层组装Janus凝集素和糖化合物来构建有组织且可控制的超分子体系结构。此外，Janus凝集素被用作生物分子连接体，将由性质不同的巨大单层囊泡制成的原细胞组织为更复杂的原组织。总之，量身定制的Janus凝集素为创建仿生基质或人造组织提供了广泛的可能性。

更新日期：2018-08-14

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