Adhesion analysis of single circulating tumor cells on a base layer of endothelial cells using open microfluidics†,Chemical Science

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Adhesion analysis of single circulating tumor cells on a base layer of endothelial cells using open microfluidics†
Chemical Science ( IF 7.6 ) Pub Date : 2018-08-13 00:00:00 , DOI: 10.1039/c8sc03027h
Sifeng Mao ₁ , Qiang Zhang ₁ , Haifang Li ₁ , Wanling Zhang ₁ , Qiushi Huang ₁ , Mashooq Khan ₁ , Jin-Ming Lin ₁

Affiliation

Circulating Tumor Cell (CTC) adhesion is essential in understanding the mechanism of metastasis. Although conventional methods for measuring adhesion strength have performed well on cell populations, a deeper insight into cell behavior demands new approaches for realizing non-destructive, high-resolution, in situ analysis of single cell adhesion. Here, we present a microfluidic method for adhesion strength analysis of single CTCs on a base layer of endothelial cells (ECs) to clarify cell-to-cell adhesion at single cell resolution. A confined flow in open space formed by a microfluidic device supplied a trypsin zone for the analysis of single cell adhesion. Tumor cell lines were used to model CTCs. This method was proved successful for extracting different types of CTCs from an endothelial cell layer to measure their adhesion strength by the time required for detachment. Moreover, we successfully uncovered the drug influence on the adhesion strength of single CTCs on ECs, which is promising in drug screening for tumor therapy. The current work reports a general strategy for cell-to-cell adhesion analysis for single cells.

中文翻译：

使用开放微流体对内皮细胞基底层上的单个循环肿瘤细胞进行粘附分析†

循环肿瘤细胞（CTC）粘附对于理解转移机制至关重要。尽管测量粘附强度的传统方法在细胞群上表现良好，但更深入地了解细胞行为需要新的方法来实现单细胞粘附的非破坏性、高分辨率、原位分析。在这里，我们提出了一种微流体方法，用于分析内皮细胞 (EC) 基层上单个 CTC 的粘附强度，以阐明单细胞分辨率下的细胞间粘附。微流体装置形成的开放空间中的受限流动为单细胞粘附分析提供了胰蛋白酶区域。肿瘤细胞系用于模拟 CTC。该方法被证明可以成功地从内皮细胞层中提取不同类型的CTC，并通过分离所需的时间来测量它们的粘附强度。此外，我们成功揭示了药物对单个CTCs对ECs粘附强度的影响，这在肿瘤治疗的药物筛选中具有广阔的前景。目前的工作报告了单细胞细胞间粘附分析的一般策略。

更新日期：2018-08-13

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