A simple, rapid, and sensitive homogeneous electrochemical bleomycin (BLM) bioassay has been successfully developed through the target-induced specific/efficient cleavage reaction. The designed probe, denoted as MB-DNA, contains both methylene blue (MB) and target recognizable sequences, and presents relatively low electrochemical signal. Upon the addition of BLM, the recognition/cleavage reaction occurs and leads to the in-situ generation of MB tag (MB-DNA-1), leading to the reduced electrostatic repulsive force. As a result, an obvious enhancement in differential pulse voltammetry (DPV) current is determined, which is relied on the amount of BLM. Thus, a turn on homogeneous electrochemical method for BLM is really achieved, and exhibits high sensitivity of 33 pM, and the shortest response time of 20 min. Furthermore, this electrochemical bioassay presents excellent sensing performance in the analysis of BLM in real samples. Comparing with other sensing strategies for BLM, this proposed electrochemical platform is just consisted of one DNA probe alone, and affords a really rapid and sensitive strategy for BLM analysis.

通过靶标诱导的特异性/有效裂解反应，已成功开发出一种简单，快速且灵敏的均质电化学博来霉素（BLM）生物测定法。被设计为MB-DNA的探针，既包含亚甲基蓝（MB），又具有靶标可识别的序列，并且呈现相对较低的电化学信号。加入BLM后，会发生识别/裂解反应，并导致MB标签（MB-DNA-1）的原位生成，从而导致静电排斥力降低。结果，确定了差分脉冲伏安法（DPV）电流的明显增强，这取决于BLM的量。因此，真正实现了BLM的均相电化学方法的开启，并显示了33 pM的高灵敏度和最短的20分钟响应时间。此外，该电化学生物测定法在实际样品的BLM分析中具有出色的传感性能。与BLM的其他传感策略相比，该拟议的电化学平台仅由一个DNA探针组成，为BLM分析提供了一种真正快速而灵敏的策略。