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Identification of Missing Proteins in Normal Human Cerebrospinal Fluid
Journal of Proteome Research ( IF 4.4 ) Pub Date : 2018-08-17 , DOI: 10.1021/acs.jproteome.8b00194
Charlotte Macron 1 , Lydie Lane 2, 3 , Antonio Núñez Galindo 1 , Loïc Dayon 1
Affiliation  

The cerebrospinal fluid (CSF) proteome data set presented herein was obtained after immunodepletion of abundant proteins and off-gel electrophoresis fractionation of a commercial pool of normal human CSF; liquid chromatography tandem mass spectrometry analysis was performed with a linear ion trap-Orbitrap Elite. We report the identification of 12 344 peptides mapping on 2281 proteins. In the context of the Chromosome-centric Human Proteome Project (C-HPP), the existence of seven missing proteins is proposed to be validated. This data set is available to the ProteomeXchange Consortium (http://www.proteomexchange.org/) with the data set identifier PXD008029.

中文翻译:

正常人脑脊液中缺失蛋白的鉴定

本文提供的脑脊髓液(CSF)蛋白质组数据集是在对大量蛋白质进行免疫耗竭并从正常人CSF的商业库中进行凝胶外电泳分离后获得的;用线性离子阱-Orbitrap Elite进行液相色谱串联质谱分析。我们报告鉴定映射到2281蛋白质的12 344肽。在以染色体为中心的人类蛋白质组计划(C-HPP)的背景下,提议验证7种缺失蛋白的存在。该数据集可用于ProteomeXchange联盟(http://www.proteomexchange.org/),其数据集标识符为PXD008029。
更新日期:2018-11-29
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