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Bioremediation and biomass production from the cultivation of probiotic Saccharomyces boulardii in parboiled rice effluent
Journal of Environmental Management ( IF 8.0 ) Pub Date : 2018-08-13 , DOI: 10.1016/j.jenvman.2018.08.027
Giana Gaboardi , Diego Gil de los Santos , Lantier Mendes , Larissa Centeno , Taiane Meireles , Samantha Vargas , Emili Griep , Arthur de Castro Jorge Silva , Ângela Nunes Moreira , Fabricio Rochedo Conceição

The parboilization of rice generates 2 L of effluent per kilogram of processed grain. Several methodologies have previously been tested with the aim of reducing the environmental impact of this effluent. The objective of this study was to evaluate the bioremediation of parboiled rice effluent supplemented with sucrose or residual glycerol from the biodiesel during the cultivation of the Saccharomyces boulardii probiotic. In the first stage of the experiment, cultures were grown in orbital shaker, and five media compositions were evaluated: 1) parboiled rice effluent; 2) effluent supplemented with 1% sucrose; 3) effluent supplemented with 3% sucrose; 4) effluent supplemented with 15 g.L−1 of biodiesel glycerol and 5) standard yeast culture medium (YM). The addition of 1% of sucrose generated the most promising results in terms of cell viability, removal of nitrogen, phosphorus and chemical oxygen demand (COD). From these results, four independent cultures were grown in a bioreactor using effluent +1% of sucrose as the medium. This assays generated a mean of 3.8 g.L−1 of biomass, 1.8 × 1011 CFU.L−1, and removal of 74% of COD and 78% of phosphorus. Therefore, the cultivation of Saccharomyces boulardii in parboiled rice effluent supplemented with 1% sucrose may represent a viable method by which the environmental impact of this effluent can be reduced while simultaneously producing probiotic culture for use in animal production.



中文翻译:

煮熟的稻米废水中益生菌酿酒酵母的生物修复和生物量生产

大米的半煮熟过程每千克加工谷物产生2升废水。先前已经测试了几种方法,目的是减少这种废水对环境的影响。这项研究的目的是评估在生化酿酒酵母益生菌的过程中,从生物柴油中补充蔗糖或残留甘油的半熟大米废水的生物修复。在实验的第一阶段,将培养物在定轨振荡器中生长,并评估了五种培养基的组成:1)煮熟的稻米出水;2)废水中补充了1%的蔗糖;3)废水中补充了3%的蔗糖;4)废水补充了15 gL -1生物柴油甘油和5)标准酵母培养基(YM)。就细胞活力,去除氮,磷和化学需氧量(COD)而言,添加1%的蔗糖产生了最有希望的结果。根据这些结果,使用流出液+ 1%的蔗糖作为培养基,在生物反应器中培养了四种独立的培养物。该测定产生平均3.8 gL -1的生物质,1.8×10 11  CFU.L -1,并去除了74%的COD和78%的磷。因此,栽培酿酒酵母 补充1%蔗糖的半熟米废水中的有机磷可以代表一种可行的方法,通过该方法可以减少废水的环境影响,同时生产用于动物生产的益生菌培养物。

更新日期:2018-08-13
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