During the regeneration of tissues and organs, growth factors (GFs) play a vital role by affecting cell behavior. However, because of the low half-life time and quick degradation of GFs, their stimulations on cells are relatively short and discontinuous. In this study, a releasing scaffold platform, consisting of polycaprolactone (PCL) nanofibers and vascular endothelial growth factor (VEGF)-encapsulated gelatin particles, was developed to extend the influence of GFs on mesenchymal stem cells (MSCs) and endothelial cells (ECs). The results showed that this kind of scaffold can direct the differentiation of MSCs to ECs and maintain the stability of the tubular structure, an indicator of the angiogenesis ability of ECs, for an extended period of time. Therefore, the results suggest the potential application of PCL/VEGF-encapsulated gelatin particles (PCL/VGPs) as a growth factor (GF)-releasing scaffold platform in vascular tissue engineering.

中文翻译：

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包含血管内皮生长因子包裹的明胶粒子的聚己内酯纳米纤维增强间充质干细胞分化和内皮细胞的血管生成。

在组织和器官的再生过程中，生长因子（GFs）通过影响细胞行为起着至关重要的作用。但是，由于半衰期短和GF的快速降解，它们对细胞的刺激相对较短且不连续。在这项研究中，开发了由聚己内酯（PCL）纳米纤维和血管内皮生长因子（VEGF）封装的明胶颗粒组成的释放支架平台，以扩展GFs对间充质干细胞（MSCs）和内皮细胞（ECs）的影响。结果表明，这种支架可以指导MSCs向ECs的分化，并在很长一段时间内保持管状结构的稳定性，而管状结构的稳定性是ECs血管生成能力的指标。所以，