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Frequency-enhanced transferrin receptor antibody-labelled microfluidic chip (FETAL-Chip) enables efficient enrichment of circulating nucleated red blood cells for non-invasive prenatal diagnosis†
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-08-09 00:00:00 , DOI: 10.1039/c8lc00650d Huimin Zhang 1, 2, 3, 4, 5 , Yuanyuan Yang 6, 7, 8, 9, 10 , Xingrui Li 6, 7, 8, 9, 10 , Yuanzhi Shi 6, 7, 8, 9, 10 , Bin Hu 6, 7, 8, 9, 10 , Yuan An 6, 7, 8, 9, 10 , Zhi Zhu 6, 7, 8, 9, 10 , Guolin Hong 6, 7, 8, 9, 10 , Chaoyong James Yang 1, 2, 3, 4, 5
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-08-09 00:00:00 , DOI: 10.1039/c8lc00650d Huimin Zhang 1, 2, 3, 4, 5 , Yuanyuan Yang 6, 7, 8, 9, 10 , Xingrui Li 6, 7, 8, 9, 10 , Yuanzhi Shi 6, 7, 8, 9, 10 , Bin Hu 6, 7, 8, 9, 10 , Yuan An 6, 7, 8, 9, 10 , Zhi Zhu 6, 7, 8, 9, 10 , Guolin Hong 6, 7, 8, 9, 10 , Chaoyong James Yang 1, 2, 3, 4, 5
Affiliation
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Fetal aneuploidy and other chromosomal aberrations affect 9 in 1000 live births. Unlike the invasive diagnosis with high risk of miscarriage, non-invasive prenatal diagnosis (NIPD) sampling from maternal blood becomes a promising way for fetal genetic screening. However, fetal cell-based NIPD has a major challenge due to the small number of fetal cells present in maternal blood. We designed a frequency-enhanced transferrin receptor antibody-labelled microfluidic chip (FETAL-Chip) for efficient enrichment and identification of circulating fetal cells, i.e., circulating nucleated red blood cells (cNRBCs) from maternal blood. The FETAL-Chip can dramatically enhance the interaction of fetal cells with antibody-coated microposts to increase the capture efficiency while minimizing nonspecific adsorption. With the help of immunostaining, we can identify cNRBCs from as little as 2 milliliter maternal blood. Various numbers of cNRBCs were detected from volunteers as early as 7 weeks after conception and throughout the entire pregnancy. Gene analysis was also carried out to confirm the fetal origin of captured cells. With easy, non-invasive and highly efficient enrichment of cNRBCs, the method presented here offers great potential for non-invasive prenatal diagnosis.
中文翻译：
频率增强的转铁蛋白受体抗体标记的微流控芯片（FETAL-Chip）可有效富集循环中的有核红细胞，用于非侵入性产前诊断†
胎儿非整倍性和其他染色体畸变影响每1000例活产中的9例。与具有高流产风险的侵入性诊断不同，从母体血液中进行非侵入性产前诊断（NIPD）采样成为胎儿基因筛查的一种有前途的方法。然而，由于母体血液中存在少量的胎儿细胞，基于胎儿细胞的NIPD面临着重大挑战。我们设计了频率增强的转铁蛋白受体抗体标记的微流控芯片（FETAL-Chip），以有效富集和鉴定循环胎儿细胞，即，来自孕妇血液的循环有核红细胞（cNRBC）。胎儿芯片可以显着增强胎儿细胞与抗体包被的微柱的相互作用，从而提高捕获效率，同时最大程度地减少非特异性吸附。借助免疫染色，我们可以从少至2毫升的母血中鉴定出cNRBC。早在受孕后7周以及整个妊娠期间，就从志愿者中检测到各种cNRBC。还进行了基因分析以确认捕获细胞的胎儿来源。通过简便，非侵入性和高效的cNRBCs富集，此处介绍的方法为非侵入性产前诊断提供了巨大的潜力。
更新日期：2018-08-09
中文翻译：
频率增强的转铁蛋白受体抗体标记的微流控芯片（FETAL-Chip）可有效富集循环中的有核红细胞，用于非侵入性产前诊断†
胎儿非整倍性和其他染色体畸变影响每1000例活产中的9例。与具有高流产风险的侵入性诊断不同，从母体血液中进行非侵入性产前诊断（NIPD）采样成为胎儿基因筛查的一种有前途的方法。然而，由于母体血液中存在少量的胎儿细胞，基于胎儿细胞的NIPD面临着重大挑战。我们设计了频率增强的转铁蛋白受体抗体标记的微流控芯片（FETAL-Chip），以有效富集和鉴定循环胎儿细胞，即，来自孕妇血液的循环有核红细胞（cNRBC）。胎儿芯片可以显着增强胎儿细胞与抗体包被的微柱的相互作用，从而提高捕获效率，同时最大程度地减少非特异性吸附。借助免疫染色，我们可以从少至2毫升的母血中鉴定出cNRBC。早在受孕后7周以及整个妊娠期间，就从志愿者中检测到各种cNRBC。还进行了基因分析以确认捕获细胞的胎儿来源。通过简便，非侵入性和高效的cNRBCs富集，此处介绍的方法为非侵入性产前诊断提供了巨大的潜力。
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