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Effect of high pressure and magnetic field treatments on stability of Candida antarctica lipase B (CALB) and lysozyme from chicken egg
Catalysis Communications ( IF 3.7 ) Pub Date : 2018-08-09 , DOI: 10.1016/j.catcom.2018.08.006
Lídia Tiggemann Prando , Marina de Souza Melchiors , Talyta Mayara Silva Torres , Vanessa Almeida de Oliveira , Josamaique Gilson Veneral , Marcos Antonio Castiani , Débora de Oliveira , J. Vladimir de Oliveira , Marco Di Luccio

The aim of this study was to treat enzymes with high pressure or magnetic field, and to evaluate their storage stability after treatment. The application of a magnetic field of 1.34 T increased the lysozyme activity about 55%, while a treatment with 0.7 T increased 80% the Candida antarctica lipase B (CALB) activity. After the magnetic treatment, the residual activity was maintained above 140% for lysozyme and CALB at 4 °C, showing good stability after treatment. Furthermore, lysozyme treated with supercritical CO2 had lower decrease on activity during storage, compared to others gases, maintaining residual activity over 100%.



中文翻译:

高压和磁场处理对鸡蛋中南极假丝酵母脂肪酶B(CALB)和溶菌酶稳定性的影响

这项研究的目的是在高压或磁场下处理酶,并评估其在处理后的储存稳定性。施加1.34 T的磁场可使溶菌酶活性增加约55%,而使用0.7 T的处理则使80%的南极假丝酵母脂肪酶B(CALB)活性增加。磁处理后,溶菌酶和CALB的残留活性在4°C时保持在140%以上,显示出良好的稳定性。此外,与其他气体相比,用超临界CO 2处理过的溶菌酶在储存过程中活性降低程度更低,残留活性保持在100%以上。

更新日期:2018-08-09
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