The sorption of five potent endocrine disruptors as representative hydrophobic organic contaminants (HOCs) namely estrone (E1), 17β-estradiol (E2), estriol (E3), 17α-ethynylestradiol (EE2) and bisphenol A (BPA) on functionalized biochar (fBC) was systematically examined, with a particular focus on the importance of π-electron-donor (phenanthrene: PHEN) and π-electron-acceptors (1,3-dinitrobenzene: DNB, p-amino benzoic acid: PABA) on sorption. Experimental results suggested that hydrogen-bond formation and π-π-electron-donor-acceptor (EDA) interactions were the dominant sorption mechanisms. The sorption of HOCs decreased as E1 > E2 > EE2 > E3 > BPA based on the Freundlich and Polanyi-Mane-models. The comparison of adsorption coefficient (K_d) normalized against hexadecane-water partition coefficient (K_HW) between HOCs and PHEN indicated strong π-π-EDA interactions. π-π interactions among DNB, PHEN and HOCs were verified by the observed upfield frequency (Hz) shifts using proton nuclear magnetic resonance (¹H NMR) which identified the specific direction of π-π interactions. UV-vis spectra showed charge-transfer bands for π-donors (PHEN and HOCs) with the model π-acceptor (DNB) also demonstrating the role of π-π EDA interactions. The role of π-electron-donor and π-electron-acceptor domains in fBC was identified at different solution pH.

五个有效的内分泌干扰物为代表的疏水性有机污染物（肝卵圆细胞）即雌酮（E1），图17的吸附β雌二醇（E2），雌三醇（E3），17 α -ethynylestradiol（EE2）和双酚A（BPA）在官能化生物炭（fBC）进行了系统检查，特别关注π电子给体（菲：PHEN）和π电子受体（1,3-二硝基苯：DNB，对氨基苯甲酸：PABA）对吸附的重要性。实验结果表明，氢键的形成和π-π-电子-给体-受体（EDA）的相互作用是主要的吸附机理。根据Freundlich和Polanyi-Mane模型，随着E1> E2> EE2> E3> BPA，HOC的吸附降低。吸附系数比较（K_d）对HOC和PHEN之间的十六烷-水分配系数（ K _HW）进行了归一化，表明存在很强的π-π-EDA相互作用。DNB，PHEN和HOC之间的π-π相互作用通过使用质子核磁共振（ ¹ H NMR）观察到的高场频率（Hz）位移进行了验证，该位移确定了π-π相互作用的特定方向。紫外-可见光谱显示了π-供体（PHEN和HOC）的电荷转移带，其中模型π-受体（DNB）也证明了π-πEDA相互作用的作用。在不同溶液pH下，fBC中的π电子给体和π电子受体域的作用得到了确认。