In this work, a ratiometric fluorescent probe (RF-probe) for highly sensitive and selective detection of uric acid was reported for the first time toward H₂O₂ based on inner filter effect (IFE) between bimetallic gold/silver nanoclusters (Au/Ag NCs) and 2,3-diaminophenazine (DAP). For this RF-probe, uric acid was degraded to allantoin and H₂O₂. Upon the addition of HRP, o-phenylenediamine (OPD) could be catalytically oxidized to DAP in the presence of H₂O₂, then the fluorescence intensity corresponding to DAP at 580 nm increased dramatically with a fluorescence quenching of BSA-Au/Ag NCs at 690 nm, resulting in a RF-probe toward uric acid. This RF-probe allowed for the sensitive detection of uric acid in range of 5.0 × 10⁻⁶ M to 5.0 × 10⁻⁵ M with a detection limit (S/N = 3) as low as 5.1 × 10⁻⁶ M. At the same time, it has been successfully used for uric acid levels detection in human serum, and the results are consistent with those of the hospital. RF-probe built may provide a ratiometric fluorescence universal platform for detection of various species involving in the production of H₂O₂ in other biological systems.

在这项工作中，一个比率荧光探针（RF-探针），用于尿酸的高灵敏度和选择性检测报道首次朝向ħ ₂ ö ₂基于双金属金/银纳米簇之间的内滤效应（IFE）（金/ Ag NCs）和2,3-二氨基吩嗪（DAP）。对于该RF探针，尿酸被降解为尿囊素和H ₂ O ₂。加入HRP后，在H ₂ O ₂存在下，邻苯二胺（OPD）可以催化氧化为DAP。，然后BSA-Au / Ag NCs在690 nm处发生荧光猝灭，对应于DAP在580 nm处的荧光强度急剧增加，从而导致了向尿酸的RF探针。该RF探针允许在5.0×10 ^-6 M至5.0×10 ^-5 M的范围内灵敏地检测尿酸，检测限（S / N = 3）低至5.1×10 ^-6M。同时，它已成功用于人体血清中尿酸水平的检测，其结果与医院的一致。所构建的RF探针可以提供用于检测与其他生物系统中的H ₂ O ₂产生有关的各种物种的比率荧光通用平台。