Ossification of the ligamentum flavum (OLF) is a common spinal disorder that causes myelopathy and radiculopathy. Non-coding RNAs (ncRNAs) are involved in numerous pathological processes; however, very few ncRNAs have been identified to be correlated with OLF. Here we compared the expression of lncRNA, mRNA, circRNA, and microRNA in OLF tissues from OLF patients and healthy volunteers through mRNA, lncRNA, and circRNA microarrays and microRNA sequencing. A total of 2,054 mRNAs, 2,567 lncRNAs, 627 circRNAs, and 28 microRNAs (miRNAs) were altered during the process of OLF. qPCR confirmed the differential expression of selected mRNAs and ncRNAs. An lncRNA-mRNA co-expression network, miRNA-mRNA target prediction network, and competing endogenous RNA (ceRNA) network of circRNA-miRNA-mRNA were constructed based on a correlation analysis of the differentially expressed RNA transcripts. Subsequently, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses for the differentially expressed mRNAs and the predicted miRNAs target genes were performed. In addition, a deregulated miRNA-19b-3p-based miRNA-circRNA-lncRNA-mRNA network was confirmed, by gain-of-function and loss-of-function experiments, to function in the process of ossification. Taken together, this study provides a systematic perspective on the potential function of ncRNAs in the pathogenesis of OLF.

对骨化黄韧带（OLF）是导致脊髓病和神经根病的常见脊柱疾病。非编码RNA（ncRNA）参与许多病理过程。但是，很少有ncRNA与OLF相关。在这里，我们通过mRNA，lncRNA和circRNA微阵列以及microRNA测序，比较了来自OLF患者和健康志愿者的OLF组织中lncRNA，mRNA，circRNA和microRNA的表达。在OLF过程中，总共改变了2,054个mRNA，2,567个lncRNA，627个circRNA和28个microRNA（miRNA）。qPCR证实了选定的mRNA和ncRNA的差异表达。基于差异表达的RNA转录本的相关性分析，构建了lncRNA-mRNA共表达网络，miRNA-mRNA目标预测网络和circRNA-miRNA-mRNA竞争内源RNA（ceRNA）网络。随后，对差异表达的mRNA和预测的miRNA目标基因进行了基因本体论（GO）和《京都议定书》。此外，通过功能获得和功能丧失实验，证实了基于失调的基于miRNA-19b-3p的miRNA-circRNA-lncRNA-mRNA网络可在骨化过程中发挥作用。综上所述，本研究为ncRNA在OLF发病机理中的潜在功能提供了系统的见解。在骨化过程中发挥作用。综上所述，本研究为ncRNA在OLF发病机理中的潜在功能提供了系统的见解。在骨化过程中发挥作用。综上所述，本研究为ncRNA在OLF发病机理中的潜在功能提供了系统的见解。