This work proposes the first method based on hydrophilic interaction liquid chromatography coupled to multiple reaction monitoring with triple stage fragmentation (HILIC-MRM³) to quantify polar organic micropollutants in complex sewage waters. A fast HILIC-MRM³ analytical method, without sample preparation except a dilution step, was developed and validated to quantify seven iodinated contrast media (ICMs) in sewage waters, namely iohexol, iomeprol, ioversol, iopamidol, diatrizoic acid, iopromide and iopentol. Several chromatographic columns and mobile phase conditions were investigated and a good separation of the ICMs was obtained with a mixed-mode column (Acclaim Mixed-mode WAX) used in HILIC conditions. The validation was performed using a synthetic matrix: the limits of quantification (LOQ) were inferior to 1 µg/L and the linearity of each compound was comprised within the [0.5–50] µg/L range. The applicability of the HILIC-MRM³ method was assessed by the analysis of several raw waters. The results highlighted the presence of ICMs in most samples, at concentrations up to several mg/L in hospital sewage waters.

这项工作提出了第一种方法，该方法基于亲水相互作用液相色谱，结合多阶段监测与三阶段碎裂的多反应监测（HILIC-MRM ³），用于定量分析复杂污水中的极性有机微量污染物。快速HILIC-MRM ³开发了一种无需稀释即可进行样品制备的分析方法，并进行了定量分析，以定量分析废水中的七种碘化造影剂（ICM），即碘海醇，碘美普尔，碘夫醇，碘帕多尔，泛影石酸，碘普罗米特和碘戊醇。研究了几种色谱柱和流动相条件，并通过在HILIC条件下使用的混合模式色谱柱（Acclaim混合模式WAX）获得了良好的ICM分离效果。使用合成基质进行验证：定量限（LOQ）低于1 µg / L，每种化合物的线性均在[0.5–50] µg / L范围内。HILIC-MRM ³的适用性通过分析几种原水来评估该方法。结果强调了大多数样品中ICM的存在，其在医院污水中的浓度高达几毫克/升。