In this work, core-shell structured magnetic metal-organic framework composites denoted as Fe₃O₄ @MIL-100(Fe) were synthesized by means of a layer-by-layer assembly method selecting Fe as metal center and 1,3,5-benzenetricarboxylic acid as organic ligand. The as-prepared material exhibited outstanding sensitivity (0.1 fmol/μL), good selectivity (1:20 and 1:50 respectively), excellent ability of size-exclusion (1: 500), fine reusability (six cycles) and great stability (two months) in enriching N-linked glycopeptides and phosphopeptides from tryptic digests of standard proteins by combining HILIC and IMAC. Moreover, it was applied into the enrichment of endogenous N-linked glycopeptides and phosphopeptides in human saliva and achieved great results (43 phosphopeptides and 39 N-linked glycopeptides), revealing its promising potential in enrichment of low-abundance endogenous N-linked glycopeptides and phosphopeptides in practical samples.

在这项工作中，表示为Fe ₃ O _4的核-壳结构磁性金属-有机骨架复合材料通过以Fe为金属中心，1,3,5-苯三甲酸为有机配体的层层组装法合成了@ MIL-100（Fe）。所制备的材料具有出色的灵敏度（0.1 fmol /μL），良好的选择性（分别为1:20和1:50），优异的尺寸排阻能力（1：500），良好的可重复使用性（六个循环）和出色的稳定性（ （两个月），通过结合HILIC和IMAC从标准蛋白的胰蛋白酶消化物中富集N-连接的糖肽和磷酸肽。此外，它还被用于人类唾液中内源性N-连接糖肽和磷酸肽的富集，并取得了丰硕的成果（43个磷酸肽和39个N-连接糖肽），显示出其在富集低丰度内源性N-连接糖肽和磷酸化肽方面的潜在潜力。实际样品中的磷酸肽。