Abstract The present work aims to prepare and stabilize zein colloidal nanoparticles using soluble soybean polysaccharide (SSPS) as a stabilizer. A simple antisolvent precipitation method was used to fabricate the zein/SSPS composite nanoparticles at pH 4.0. The results showed that the SSPS-stabilized zein nanoparticles (size around 200 nm, PDI below 0.2) did not aggregate or precipitate at pH 2.0–8.0. And they were also relatively stable for elevated ionic strength and high temperature. Affected by the SSPS coating, the surface hydrophobicity of the zein nanoparticles decreased over a wide pH range. Conditions of forming zein/SSPS nanoparticles were successfully used to encapsulate hydrophobic quercetin (Q). The encapsulation efficiency was greatly improved to 82.5% after SSPS coating, compared to 59.2% for the zein without coating. Besides, the photochemical stability and ABTS+ scavenging ability of Q in zein/SSPS composite nanoparticles were significantly enhanced. This study demonstrates that these composite nanoparticles can be used as all-natural delivery systems for bioactive molecules in food and pharmaceutical formulations.

中文翻译：

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包覆槲皮素的可溶性大豆多糖包覆稳定玉米醇溶蛋白纳米粒的制备

摘要 本工作旨在以可溶性大豆多糖 (SSPS) 作为稳定剂制备和稳定玉米醇溶蛋白胶体纳米颗粒。使用简单的反溶剂沉淀法在 pH 4.0 下制备玉米醇溶蛋白/SSPS 复合纳米粒子。结果表明，SSPS 稳定的玉米醇溶蛋白纳米颗粒（尺寸约为 200 nm，PDI 低于 0.2）在 pH 2.0-8.0 时不会聚集或沉淀。并且它们对于升高的离子强度和高温也相对稳定。受 SSPS 涂层的影响，玉米醇溶蛋白纳米颗粒的表面疏水性在很宽的 pH 范围内降低。形成玉米醇溶蛋白/SSPS 纳米粒子的条件已成功用于封装疏水性槲皮素 (Q)。SSPS 包衣后的包封率大大提高到 82.5%，而没有包衣的玉米蛋白则为 59.2%。除了，Q在玉米醇溶蛋白/SSPS复合纳米粒子中的光化学稳定性和ABTS+清除能力显着增强。这项研究表明，这些复合纳米粒子可用作食品和药物制剂中生物活性分子的全天然递送系统。