Rational Design of Amphiphilic Peptides and Its Effect on Antifouling Performance,Biomacromolecules

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Rational Design of Amphiphilic Peptides and Its Effect on Antifouling Performance
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-08-03 00:00:00 , DOI: 10.1021/acs.biomac.8b00587
Sheng Long Gaw ₁ , Gowripriya Sakala ₂ , Sivan Nir ₂ , Abhijit Saha ₂ , Zhichuan J. Xu ₁ , Pooi See Lee ₁ , Meital Reches ₂

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Biofouling, the unwanted adhesion of organisms to surfaces, has a negative impact on energy, food, water, and health resources. One possible strategy to fight biofouling is to modify the surface using a peptide-based coating that will change the surface properties. We reveal the importance of rational design and positioning of individual amino acids in an amphiphilic peptide sequence. By just manipulating the position of the amino acids within the peptide chain having the same chemical composition, we improved the antifouling performance of an amphiphilic peptide-based coating, Phe(4-F)-Lys-DOPA, by 30%. We have judiciously tailored the peptide configurations to achieve the best antifouling performance by (i) positioning the amino acid lysine adjacent to the DOPA moiety in the linear peptide chain for better adhesion, (ii) having a linear fluorinated N-terminal to improve the packing density of the film by straightening the peptide chain, and (iii) placing DOPA at the C-terminal. We have also compared the antifouling performances of amphiphilic, hydrophobic, hydrophilic, and alternately arranged peptides. Our results show a reduction of ∼80% in bacterial adhesion for an amphiphilic peptide-coated surface when compared to a bare titanium surface. This work provides important strategic design guidelines for future peptide-related materials that have effective antifouling properties.

中文翻译：

两亲肽的合理设计及其对防污性能的影响。

生物污垢是有机物与表面的有害附着，会对能源，食物，水和健康资源产生负面影响。对抗生物污垢的一种可能策略是使用会改变表面特性的基于肽的涂层来修饰表面。我们揭示了两亲性肽序列中单个氨基酸的合理设计和定位的重要性。通过仅操纵具有相同化学组成的肽链中氨基酸的位置，我们将两亲性肽基涂层Phe（4-F）-Lys-DOPA的防污性能提高了30％。我们通过（i）将氨基酸赖氨酸与线性肽链中的DOPA部分相邻放置，明智地调整了肽的构型，以实现最佳的防污性能。（ii）具有直链的氟化N端以通过拉直肽链来改善薄膜的堆积密度，以及（iii）将DOPA置于C端。我们还比较了两亲，疏水，亲水和交替排列的肽的防污性能。我们的结果表明，与裸露的钛表面相比，两亲性肽涂层表面的细菌粘附力降低了约80％。这项工作为具有有效防污性能的未来肽相关材料提供了重要的战略设计指南。我们的结果表明，与裸露的钛表面相比，两亲性肽涂层表面的细菌粘附力降低了约80％。这项工作为具有有效防污性能的未来肽相关材料提供了重要的战略设计指南。我们的结果表明，与裸露的钛表面相比，两亲性肽涂层表面的细菌粘附力降低了约80％。这项工作为具有有效防污性能的未来肽相关材料提供了重要的战略设计指南。

更新日期：2018-08-03

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